Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-03-29
William J. Liu
Peipei Liu
Wenwen Lei
Zhiyuan Jia
Xiaozhou He
Weifeng Shi
Yun Tan
Shumei Zou
Gary Wong
Ji Wang
Feng Wang
Gang Wang
Kun Qin
Rongbao Gao
Jie Zhang
Min Li
Wenling Xiao
Yuanyuan Guo
Ziqian Xu
Yingze Zhao
Abstract： Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and posed a tremendous threat to global public health. However, the origins of SARS-CoV-2, the causative agent of COVID-19, remain to be determined. It has been reported that a certain number of the early case clusters had a contact history with the Huanan Seafood Market. Therefore, surveillance of SARS-CoV-2 within the market is of vital importance. Herein, we presented the SARS-CoV-2 detection results of 1380 samples collected from the environment since 1st Jan and animals since 18th Jan within the market in early 2020. By SARS-CoV-2-specific RT-qPCR, 73 environmental samples tested positive for SARS-CoV-2 and three live viruses were successfully isolated. The viruses from the market shared nucleotide identity of 99.99% to 100% with the human isolate HCoV-19/Wuhan/IVDC-HB-01/2019. The A lineage (8782T and 28144C), as the likely ancestral SARS-CoV-2 lineage, was found in an environmental sample. No virus was detected in the animal swabs covering 18 species of animals in the market. The RNA-seq analysis of SARS-CoV-2 positive/negative environmental samples showed the abundance of different vertebrata genera. In summary, this study provided convincing evidence of the prevalence of SARS-CoV-2 in the Huanan Seafood Market during the early stage of COVID-19 outbreak.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Virology Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2021-07-28
Qihui Wang
Hua Chen
Yi Shi
Alice C. Hughes
William J. Liu
Jingkun Jiang
George F. Gao
Yongbiao Xue
Yigang Tong
Abstract： The origin of SARS-CoV-2 remains elusive. Understanding how, when, and where SARS-CoV-2 was transmitted from its natural reservoir to human beings is crucial for preventing future coronavirus outbreaks. With the lessons learned from the endless battle with pathogens and accumulated research data with regard to the origin and intermediate host, we present multiple potential locations as the natural reservoir of SARS-CoV-2.
Peer Review Status:Awaiting Review
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