Subjects: Law >> Legal professional ethics submitted time 2024-01-23
Abstract： Big data legal supervision is a hot topic at the moment and the key to a new round of prosecutorial system reform. Big data legal supervision has put forward new requirements for procuratorial organs, and they need to have a grasp of its basic theories. As a new thing, big data legal supervision is still booming and will inevitably encounter problems in various aspects. The most prominent and controversial aspects are data, algorithms and systems. For big data legal supervision to play its intended role, improvements need to be made jointly from multiple perspectives. In view of the current problems and based on the actual situation in our country, this article proposes some improvement measures to provide some reference for the introduction of relevant regulations.
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Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：丁酸梭菌（Clostridium butyricum）具有调节肠道微生态平衡、产生消化酶和维生素等营养物质、提高动物免疫力、抗细胞凋亡、抗肿瘤等多种生理功能。此外，丁酸梭菌属于厌氧芽孢杆菌，对饲料加工过程中的高温、高压以及动物胃肠道环境有较好的耐受性。因此，丁酸梭菌是一种适合在畜禽生产中添加的益生菌。本文总结了丁酸梭菌的生物学特性、生理功能和对畜禽生产的影响及其作用机理，为丁酸梭菌的进一步研究和开发应用提供参考。
Subjects: Biology >> Bioengineering submitted time 2018-11-17 Cooperative journals: 《中国生物工程杂志》
Abstract：α-酮戊二酸（α-ketoglutaric acid, α-KG）是谷氨酸脱氨基的酮酸产物，作为一种重要的有机酸广泛用于食品、医药、精细化工等领域。为提高L-氨基酸脱氨酶全细胞催化法合成α-KG的效率及产量，首先通过优化全细胞催化剂制备条件及全细胞转化反应条件，包括发酵过程中的温度、pH、诱导剂浓度，诱导剂添加时刻、诱导时间等；全细胞转化过程中的温度、pH、细胞量、转化时间。各个条件优化后以200 g·L-1谷氨酸钠为底物时，产量最终提高了54.9 %，摩尔转化率为39.6 %。其次，通过定点饱和突变对L-氨基酸脱氨酶进行定向进化以提高其催化能力。经过多次突变、筛选，最优突变体E.coli BL21-pET-20b(+)-pm1152催化200 g·L-1谷氨酸钠生成α-KG最高产量为 100.9 g·L-1，摩尔转化率为64.7 %，较最初对照菌株提高了66.3 %。结果表明，条件优化和饱和突变可有效提高重组大肠杆菌全细胞转化合成α-KG的能力。
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