Subjects: Biology >> Botany submitted time 2022-12-29 Cooperative journals: 《广西植物》
Abstract：
In order to find out the optimum harvest time of Polygala fallax, the present study analyze the root morphology, biomass, medicinal components, nutritional components and mineral elements of Polygala fallax roots during the 1~5 cultivating year by national standards and other methods. The results were as follows: (1) The morphology and biomass of Polygala fallax root increasd slowly during the first two cultivating year, and increased rapidly during the 2~3 cultivating year, however the parameters tended to be flat during the 3~5 cultivating year; (2) The content of different medicinal ingredients varied significantly within the cultivating year, polysaccharides reached to the peak at the first year, saponins reached to the peak in the second year, and flavonoids reached to the peak at the fourth year; (3) The mineral elements showed various behavior among cultivating years, the contents of Ca, Mg, Mn and Cu accumulated to the maximum in the third year, content of Fe decreased with increasing cultivating year, and the content of Pb increased with increasing cultivating year. (4) Crude protein increased first and then decreased, and the same change trands were observed in content of total ash and crude fat, both of which decreased as cultivating year increasing. The results show that Polygala fallax reaches a high yield in the third cultivating year, and most of the quality indicators in the roots of 4~5 year at the lowest value, and the third year is the ideal harvest period for Polygala fallax, which provide technical guidance for high-yield and good quality of Polygala fallax. The results of the study elucidate the dynamics accumulation of Polygala fallax during different cultivating year and reveal the optimal harvest time of Polygala fallax.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Flavonoid-C- glycosides, as one of the effective components in Premna fulva, have the effect of antigen-induced arthritis. The enrichment and purification process of five flavonoid-C-glycosides as in P. fulva stems is preferred. Through static adsorption and desorption experiments on 13 kinds of macroporous resins with five flavonoid-C- glycosides in P. fulva stems as the index,a suitable macroporous resin was selected, and the results were tested by the HPLC, and then the orthogonal process was used to optimize the enrichment and purification conditions. The results showed that XAD-16N macroporous resin has a good adsorption and desorption effect on the five flavonoid -C-glycosides in P. fulva stems. Through single factor screening and orthogonal process optimization, the best enrichment and purification process is finally determined as follows: the loading concentration is 0.5 times the original extract diluted, the loading flow rate is 2 BV穐-1, and the eluent concentration is 60 % ethanol. The content of five flavonoid-C-glycosides increased from 1.73 % (in the original plant) to 14.5 %, an increase of 7.38 times. The results showed that the selected extraction process is stable and feasible, and the selected macroporous resin has a good separation and purification effect, which provides a reference for the drug development of P. fulva stems.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-06-17 Cooperative journals: 《广西植物》
Abstract：植物内生真菌与宿主植物互惠共生，可提高宿主植物的营养吸收、抗逆境能力以及刺激宿主植物活性物质的产生，是植物病害生物防治领域的重要研究内容之一。植物内生真菌种类繁多，通过ITS 基因序列扩增和测序分析，结合传统的形态学鉴定方法，可快速、有效鉴定内生真菌。本文采用传统形态学方法结合rDNA-ITS 序列分析，对我国重要药用植物罗汉果中的内生真菌进行鉴定，并研究其多样性，以期为后期探究内生真菌与罗汉果互作关系奠定基础。采用组织培养法分离内生真菌，从罗汉果健康植株中共分离得到150 株内生真菌，包括罗汉果雌株中的内生真菌96 株和罗汉果雄株中的内生真菌54 株。其中，122 株内生真菌经形态学结合rDNA-ITS 序列分析鉴定为9 个属，均归属为子囊菌门，包含座囊菌纲（Dothideomycetes）和子囊菌纲（Sordariomycetes）。其中座囊菌纲（Dothideomycetes）真菌包含3 科、3 属；子囊菌纲（Sordariomycetes）真菌包含6 科、6 属。优势属为刺盘孢属（Colletotrichum）和镰刀菌属（Fusarium）。罗汉果雌、雄植株不同组织中内生真菌的定殖率及分离率的变化规律均不相同，雌株中以根中内生真菌的定殖率和分离率最高，叶片中的最低；而在雄株中以叶片中的定殖率和分离率最高，根中的最低。另外，不同菌株在雌、雄两种植株的不同组织中的分布均不同，结合内生真菌群落组成的相似性比较结果，表明部分内生真菌对罗汉果雌株和雄株，以及同一植株中的不同组织均具有偏好性。不同组织中内生真菌的多样性指数在0.11-0.69 的范围，其中雌株根部的内生真菌多样性指数最高。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： Weeds in paddy field cause severe damage to rice (Oryza sativa L.) production. Use of rice allelopathy is considered the most environmentally friendly approach for weed control. Therefore, understanding rice alleopathy and its key allelochemicals has particularly ecological significance. Momilactones A and B are key allelochemicals produced by rice plants, and momilactone B is the most effective natural phytotoxin with great potential to be used as natural herbicides. Momilactone B can be released into rice rhizosphere and inhibit the germination and growth of barnyardgrass and other plants. It inhibited the root and hypocotyl growth of seedlings of cress and lettuce at concentrations ≥3 nmol·mL-1. Moreover, momilactone A and B are phytoalexins showing antifungal activity against blast pathogen Magnaporthe grisea. This paper reviewed important aspects in various fields of momilactone research, including its characteristics, allelopathy, biosynthesis pathway, detection, synthesis method and influencing factors. Some important issues in momilactone research including rapid and sensitive detection, induced factors and chemical synthesis are also discussed.
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