分类: 物理学 >> 核物理学 提交时间: 2017-08-22
摘要: The Woods-Saxon basis has been suggested to replace the widely used harmonic oscillator basis for solving the relativistic mean field (RMF) theory in order to generalize it to study exotic nuclei. As examples, relativistic Hartree theory is solved for spherical nuclei in a Woods-Saxon basis obtained by solving either the Schro ̈dinger equation or the Dirac equation (labelled as SRHSWS and SRHDWS, respectively and SRHWS for both). In SRHDWS, the negative levels in the Dirac Sea must be properly included. The basis in SRHDWS could be smaller than that in SRHSWS which will simplify the deformed problem. The results from SRHWS are compared in detail with those from solving the spherical relativistic Hartree theory in the harmonic oscillator basis (SRHHO) and those in the coordinate space (SRHR). All of these approaches give identical nuclear properties such as total binding energies and root mean square radii for stable nuclei. For exotic nuclei, e.g., 72Ca, SRHWS satisfactorily reproduces the neutron density distribution from SRHR, while SRHHO fails. It is shown that the Woods-Saxon basis can be extended to more complicated situations for exotic nuclei where both deformation and pairing have to be taken into account.
分类: 物理学 >> 核物理学 提交时间: 2017-08-22
摘要: We discuss spin and pseudo-spin symmetry in the spectrum of single nucleons and single anti- nucleons in a nucleus. As an example we use relativistic mean field theory to investigate single anti-nucleon spectra. We find a very well developed spin symmetry in single anti-neutron and single anti-proton spectra. The dominant components of the wave functions of the spin doublet are almost identical. This spin symmetry in anti-particle spectra and the pseudo-spin symmetry in particle spectra have the same origin. However it turns out that the spin symmetry in anti-nucleon spectra is much better developed than the pseudo-spin symmetry in normal nuclear single particle spectra.
分类: 物理学 >> 普通物理:统计和量子力学,量子信息等 提交时间: 2017-05-02
摘要: As there is no round optical grating and magnetic grating with large diameter that is over 0.5m in the market, a measuring system adopting dual magnetic sensors based on FPGA is proposed in this paper to measure the position/displacement and velocity of the rotating outer-rotor motor. Through using strip-type magnetic grating to stick around the outer rotor over half circumstance, the proposed system is able to produce continuous position pulse signals by switching the signals of dual magnetic sensors. The system core chip distinguishes, subdivides and filters the signals from two sensors, judging the operational state of the sensor and generating the dual magnetic sensor switching signals. It can switches the sensor signals in real time during motor operation and measures the position/displacement and velocity of motor in a precise manner. Moreover, the position/displacement, operating time and positive/negative rotation can be displayed through man-machine interactive unit. The experimental results show that arc-second control accuracy of the outer-rotor torque motor can be achieved by using the proposed system to measure the speed and position/ displacement of the outer-rotor torque motor as the feedback signal through the motion control system of motor.
提交时间: 2017-05-02
摘要: This study reports a viable method for the synthesis of Ti2SC phase using iron disulfide or iron sulfide as sulfur source and a post-treatment with acid. The reaction routes to Ti2SC phases starting from two different chemical compositions of 2Ti-FeS2-2Ti
分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: Rv3899c, a hypothetical protein from Mycobacterium tuberculosis that is conserved within the mycobacteria, is predicted to be secreted and has been found in culture filtrates. Here, Rv3899c has been cloned, expressed in Escherichia coli and purified using standard chromatographic techniques. The hanging-drop vapour-diffusion method with PEG 3350 as a precipitant was used to crystallize the protein. N-terminal sequencing results showed that the amino-acid sequence of the crystallized protein began with GATAG, indicating that it is a fragment containing residues 184-410 of Rv3899c. Rv3899c(184-410) crystals exhibited the symmetry of space group P2(1)2(1)2(1), with unit-cell parameters a = 49.88, b = 54.72, c = 75.52 angstrom, = = = 90 degrees, and diffracted to a resolution of 1.90 angstrom.
提交时间: 2017-05-02
摘要: V2AlC belongs to a family of ternary nano-laminate alloys known as the MAX phases, which exhibit a unique combination of metallic and ceramic properties. In this work V-Al-C coatings with deposited [V, Al][2]C nano-crystallines and amorphous phase were ma
提交时间: 2017-05-02
摘要: Porous Ti2AlN ceramic, which was measured to be 3.23 g/cm3 about 74.9% of its theoretical value, was successfully synthesized and simultaneously consolidated from starting raw materials of Ti, Al, and TiN powders with a near-stoichiometric molar ratio of
分类: 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间: 2016-05-12
摘要: The biotin biosynthesis pathway is an attractive target for development of novel drugs against mycobacterial pathogens, however there are as yet no suitable inhibitors that target this pathway in mycobacteria. 7-Keto-8-aminopelargonic acid synthase (KAPA synthase, BioF) is the enzyme which catalyzes the first committed step of the biotin synthesis pathway, but both its structure and function in mycobacteria remain unresolved. Here we present the crystal structure of Mycobacterium smegmatis BioF (MsBioF). The structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS), the E. coli homologue of BioF. To investigate the influence of structural characteristics on the function of MsBioF, we deleted bioF in M. smegmatis and confirmed that BioF is required for growth in the absence of exogenous biotin. Based on structural and mutagenesis studies, we confirmed that pyridoxal 5'-phosphate (PLP) binding site residues His129, Lys235 and His200 are essential for MsBioF activity in vivo and residue Glul 71 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo. The structure and function of MsBioF reported here provides further insights for developing new anti-tuberculosis inhibitors aimed at the biotin synthesis pathway. (C) 2014 Elsevier Ltd. All rights reserved.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-05
摘要: DNA polymerase III (DNA pol III) is a multi-subunit replication machine responsible for the accurate and rapid replication of bacterial genomes, however, how it functions in Mycobacterium tuberculosis (Mtb) requires further investigation. We have reconstituted the leading-strand replication process of the Mtb DNA pol III holoenzyme in vitro, and investigated the physical and functional relationships between its key components. We verify the presence of an alpha beta(2)epsilon polymerase-clamp-exonuclease replicase complex by biochemical methods and protein-protein interaction assays in vitro and in vivo and confirm that, in addition to the polymerase activity of its a subunit, Mtb DNA pol III has two potential proofreading subunits; the alpha and epsilon subunits. During DNA replication, the presence of the beta(2) clamp strongly promotes the polymerization of the alpha beta(2)epsilon replicase and reduces its exonuclease activity. Our work provides a foundation for further research on the mechanism by which the replication machinery switches between replication and proofreading and provides an experimental platform for the selection of antimicrobials targeting DNA replication in Mtb.