Subjects: Biology >> Biochemistry submitted time 2023-02-24
Abstract： This study aimed to explore the effect of massage on the urine proteome of healthy people. In this study, participants underwent 1-hour whole body massage. Urine samples were collected at 0, 2, and 24h after the massage and urine proteins were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Compared with the control (before massage), 41 differential proteins were identified in the group 2h after the massage, the random mean number of differentially produced proteins was 11 with 73% confidence, and the biological process of protein enrichment was catecholamine biosynthesis, which was related to the promotion of metabolism and the regulation of neural activities. While 29 differential proteins were identified in the group 24h after the massage, the random average number of differential proteins produced was 10, with the confidence of the difference decreased to 65%, and the effective biological process could not be enriched at this time. The results suggested that the difference in urine protein was greater at 2h after the massage and gradually decreased at 24h after the massage. The proteome of urine may reflect changes in the body following minor massage stimuli, providing a potential way to evaluate the effects of massage therapy.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-04-04
Abstract：
[Objective] The changes in the immune system in urine proteome were observed by injecting bovine serum albumin and aluminum hydroxide adjuvant into rats.
[Methods] In this study, bovine serum albumin and aluminum hydroxide adjuvant were injected into rat thigh muscle, urine was collected, differential proteins were identified by liquid chromatography-mass spectrometry (LC-MS / MS), and biological pathways of differential proteins were analyzed by IPA software to observe the changes of the immune system in rat urinary protein.
[Results] Fifteen rats were intramuscularly injected with normal saline, aluminum hydroxide adjuvant, bovine serum albumin, aluminum hydroxide adjuvant, and bovine serum albumin (BSA) mixture to construct the models of the control group, adjuvant group, BSA group, and mixed group. Comparing the different proteins between different groups to get the relevant biological pathways, it was found that adjuvants can be observed in urine to help bovine serum albumin stimulate the immune system to respond earlier. It was also observed in urine that the mixed group successively stimulated immune-related pathways such as inflammatory response, T cell activation, antigen-presenting cell-related pathways, and B cell-related pathways.
[Discussion] We can observe the changes in the immune system from urine proteome in the early stage, which provides some new clues and a basis for future research on the immune system and even accelerates the research and development of a vaccine.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-04-01
Abstract：目的：怀孕是身体生理状态产生极大变化的过程，正常的妊娠过程对保证胎儿发育正常和健康的母体非常重要，我们试图探索尿蛋白组变化能否反映妊娠过程。 方法：我们分别收集孕鼠妊娠0天、2天、12天、14天、16天、18天和20天的尿样，通过LC-MS高通量质谱分析不同时间的尿蛋白组，分别与妊娠第0天比较得到的差异蛋白进行生物学通路分析。 结果：妊娠第2天尿蛋白组的变化与胚胎着床、胎盘的分化相关的通路，妊娠后期（E16和E18），尿富集到与器官发育相关的通路，主要是肺发育相关的通路。有趣的是，据之前的报道，尿里发现着床相关的通路的时间点刚好在大鼠着床期内；大鼠肺发育主要是在妊娠后期(E11-E21)，我们发现尿里肺发育相关的通路也与这一时期相符。此外，从妊娠后期到分娩，发现凝血系统相关的过程不断显著，与之前报道过的妊娠过程中凝血功能不断增强的趋势一致。 结论: （1）尿蛋白组可以反映正常妊娠过程；（2）尿可能成为监测妊娠过程的有效策略。
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-14
Abstract：
Objective This study focuses on the most important concern of surgeons - whether they resected all the tumors. Urine may reflect early changes associated with physiological or pathophysiological processes. Based on the above ideas, we have conducted some experiments to explore changes in the urine proteome urine proteomes between tumor-bearing mice to tumor-resected mice. Method The tumor-bearing mouse model was established with MC38 mouse colon cancer cell, and the mice were divided into the healthy control group, tumor complete resection group, and the tumor non-resection group. Urine was collected on the 7 days and 30 days after resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome and then analyze differential proteins and biological pathways. Results (1) 7 days after the tumor removal surgery between the complete resection group and the non-resection group, there are 20 differential proteins that can distinguish the two groups. The biological process includes circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, heterophilic cell#2;cell adhesion via plasma membrane cell adhesion molecules. (2) 30 days after the tumor removal surgery between the complete resection group and the non-resection group, there are 33 differential proteins that can distinguish the two groups. The biological process includes cell adhesion, complement activation, the alternative pathway, immune system process, angiogenesis. (3) There was no significant difference between the two groups at 30 days after the tumor removal surgery between the complete resection group and the healthy control group. Conclusion The changes of urine proteome can reflect tumor with surgical removal or not.
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Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-11
Abstract：
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Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Pharmacology submitted time 2022-03-09
Abstract：
[Objective] The drug consistency evaluation of atorvastatin by urine proteomic analysis.
[Methods] Intragastric administration rat models were established by atorvastatin from two companies. Urine samples were collected from rats before and after intragastric administration. Urine proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and the biological pathway of the differential proteins was analyzed by DAVID database.
[Results] The differential proteins were selected by comparing the after intragastric administration to before intragastric administration. A total of 116 differential proteins were identified in group A and 66 differential proteins in group B, and 24 proteins were identified in common between two groups. These differential proteins tend to be involved in biological pathways such as cell adhesion, negative regulation of endopeptidase activity and proteolysis.
[Conclusions] For two kind of atorvastatin, we can detect small differences in urinary protein between them, which confirms the sensitivity of urinary protein and suggests that urine proteomics has great potential for the Drug consistency evaluation.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-03
Abstract：
Abstract：
[Objective] To investigate the effect of hypoxia on urine proteome in rats,and to find the changes of urine protein related to hypoxia stress.
[Methods] In this study,liquid chromatography-mass spectrometry (LC-MS/MS) was used to screen the urine differential proteins of rats with 12 and 24h hypoxia and analyze the biological pathway to observe the changes of urine protein in rats with acute hypoxia stress.
[Results] We found that the urine proteome could clearly distinguish the normoxic and hypoxic samples, and the differential proteins were also enriched in biological pathways related to hypoxic stress,such as antioxidant stress,glycolysis,complement and coagulation cascade.
[Conclusions] Urine proteome can reflect the significant changes after acute hypoxic stimulation, which is helpful for the detection of hypoxia.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-02-28
Abstract：
Hypertension is a multifactorial complex disease related to genetic and environmental factors, which has become the most serious public health problem. Despite progress in antihypertension, there are limitations in the diagnosis and treatment of hypertension due to the lack of relevant molecular mechanisms. Urinary proteome has the potential to provide new research strategies for complex diseases involving multiple biological pathways and organs. In this study, the stroke-prone spontaneously hypertensive rats (SHRSP) were used. Urine samples were collected at months 1, 4, 8, 10, 12, and 14 during hypertension development. Dynamic urine proteomes were analyzed by using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Considering that the degree of disease progression may be different, each rat was compared before and after to screen for differential proteins, and functional annotation analysis was performed based on the differential proteins to have a better understanding of the pathological mechanisms involved in hypertension progression. During hypertension development, urine proteins were enriched some important pathways, such as renin-angiotensin signaling response to oxidative stress, aldosterone signaling in epithelial cells, renin-angiotensin signaling, IL-12 signaling and production in macrophages, apelin adipocyte signaling pathway, calcium transport I, STAT3 pathway, and glucocorticoid receptor signaling. These pathways were reported to be involved in the pathological mechanisms of hypertension, and might provide new clues for antihypertension drug target research. This study demonstrates that urinary proteomics can reflect the pathological processes associated with hypertension. This suggests that urine proteomic approaches may be used to investigate the mechanisms associated with hypertension and to find new drug targets of hypertension, and has the potential to provide personalized antihypertensive strategies with optimal efficacy.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-01-09
Abstract： [Objective] Through the liver cancer-inflammatory cancer model induced by DEN, observe the dynamic change process of urine proteome at different stages of the disease. [Methods] In this study, a rat liver cancer-inflammatory cancer model was constructed by intraperitoneal injection of DEN, urine was collected weekly, and differential proteins were identified by liquid chromatography coupled with mass spectrometry (LC-MS/MS), and the biological pathways of differential proteins were analyzed using IPA software. To observe the changes in urine protein at different stages of the disease in the rat liver cancer-inflammatory cancer model. [Results] Fifteen experimental rats were induced by DEN to construct different stages of the hepatitis-cirrhosis-liver cancer disease model, and each rat had a different disease progression due to individual differences. Each rat has more differential proteins at different disease time points. The differential proteins of different rats are enriched in the same biological pathways related to liver injury, inflammatory lesions, tumor occurrence, and potential therapeutic targets of the tumor. [Discussion] We can observe different changes of urine proteome in each stage of the liver cancer inflammatory cancer model, and different rats will have different disease progression due to individual differences, which suggests that we need to pay more attention to the individualization and accuracy of detection in the future. "
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2021-12-01
Abstract： Abstract: [Objective] Urine proteome reflects the physiological changes of the body sensitively, which provide some clues for the development of colorectal cancer caused by inflammation. [Method] In this study, liquid chromatography coupled with mass spectrometry (LC-MS/MS) was used to screen the urine differential proteins in a mouse model of AOM/DSS-induced colorectal cancer caused and analyze the biological pathways, to explore the process of this mouse model and find the pattern in the biological pathways. [Results] (1) The changes of urine proteome can reflect the pathophysiological changes in this mouse model. There are some common biological pathways related to inflammation and tumors: acute phase response, cell adhesion, innate immune response, positive regulation of protein kinase B signaling, positive regulation of B cell activation, circadian rhythm, immune system process. (2) The disease progression of experimental mice is different, and the urine proteome of mice at different stages of progression is different. The research method of independent analysis of a single mouse used in this study can provide clues to the mouse disease process, and also provide a reference for future precision medicine. "
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2021-06-25
Abstract： "
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2021-04-08
Abstract： "
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2020-02-28
Abstract： 肺癌作为一种严重威胁人类生命健康的疾病，尽管治疗方法种类较多，但如何把握治疗时机，为患者确定最有效的治疗用药仍然是一个难题。尿液蛋白质组可以敏感的反映机体的生理变化，有希望反映药物治疗后机体的病理或生理变化。为了研究晚期肺癌患者使用不同的药物进行治疗时其尿液蛋白质组的变化情况，通过收集晚期肺癌患者治疗前后的不同时间的尿液样本，使用液相色谱联用质谱（LC-MS / MS）来检测其尿蛋白组的变化。比较结果发现，当不同的患者使用不同药物治疗后，差异蛋白的变化各不相同，其差异蛋白反映的生物学过程变化和患者的临床表现变化较为一致。这项研究结果表明，（1）能够通过尿蛋白的变化反映不同药物治疗后患者的病理生理变化；（2）当不同患者使用相同药物治疗后，尿蛋白的变化能够反映出患者体内不同的病理生理变化，且和患者的临床病情评估结果较为一致。这些发现有可能为辅助临床治疗提供重要信息。
Peer Review Status:Awaiting Review
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