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  • Research on the Construction of Knowledge Graph in the Context of Landscape Poem from the Perspective of Digital Humanities:Take Parrot Island Poetry as an Example

    Subjects: Library Science,Information Science >> Indexing and Cataloging submitted time 2024-01-10 Cooperative journals: 《文献与数据学报》

    Abstract: [Purpose/significance]Introducing landscape imagery into the field of poetry and constructing a knowledge graph can help refine the granularity of poem resources in the digital humanities domain while expanding the breadth of digital humanities research in poem. [Method/process] Facing landscape poetry, this paper takes Parrot Island poetry as the specific case object, constructs its ontology model and knowledge graph, semantically correlates landscape poetry knowledge through multiple dimensions such as poetry, landscape, characters, space, time, and events, and visualizes the semantic relationship of knowledge graph, so as to realize the knowledge discovery research of Parrot Island poetry. [Result/conclusion] By conducting empirical research through case studies in this paper, we demonstrate that constructing a knowledge graph for landscape poem not only facilitates the standardized and systematic organization of poem resources but also enables deeper exploration of poem contents. Furthermore, it provides valuable insights for the development of regional distinctive resources, with poem being representative of such resources.

  • Chloroplast genome capture history and genetic diversity of Camellia sinensis var. sinensis cv. ‘Liupao’

    Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》

    Abstract: Camellia sinensis var. sinensis cv. ‘Liupao’ is the main raw material for making Liupao tea. To explore the genetic background of C. sinensis var. sinensis cv. ‘Liupao’, especially its phylogenetic position and evolutionary history, 27 and five individuals of C. sinensis var. sinensis cv. ‘Liupao’ and C. costata were sampled for genome skimming and transcriptomic sequencing. The phylogenetic relationship between C. sinensis var. sinensis cv. ‘Liupao’ and other Camellia was reconstructed, the divergence time of the chloroplast genome sequences of C. sinensis var. sinensis cv. ‘Liupao’ was estimated, and the genetic diversity was investigated. The results were as follows: (1) On the chloroplast tree, 27 samples of C. sinensis var. sinensis cv. ‘Liupao’ were divided into two distantly-related groups, one was nested within a clade mainly consisting of C. sinensis, being interspersed among other cultivated C. sinensis, while the other group formed as a well supported lineage that was most closely-related to and nested within C. costata; On the nuclear gene tree, however, all the 27 samples formed a clade with other C. sinensis as well as some other Camellia species with generally unresolved relationships among them. Despite the lack of resolution in this clade, it was definitely far separated from C. costata; The cytonuclear phylogenetic conflict suggested once ancient introgression hybridization of C. costata with the ancestor of some C. sinensis var. sinensis cv. ‘Liupao’ individuals so that the latter captured the chloroplast genome of the former. (2) The time estimate indicated that the introgression hybridization event occurred in the Quaternary period, ca. 1.55 million years ago, long before the history of tea cultivation and production by humans. (3) In addition, both the chloroplast genomes and nuclear genes revealed that the C. sinensis var. sinensis cv. ‘Liupao’ had high haplotype diversity, possessing high evolutionary potential. This study could provide important references for the germplasm protection, breeding, and utilization of high-quality C. sinensis var. sinensis cv. ‘Liupao’ strains.

  • Research on Intelligent Question and Answer of Intangible Cultural Heritage Resources in the Yellow River Basin Based on Knowledge Graph

    Subjects: Library Science,Information Science >> Indexing and Cataloging submitted time 2023-11-07 Cooperative journals: 《文献与数据学报》

    Abstract: [Purpose/significance]The use of digital technology to empower the in-depth excavation of intangible cultural heritage resources and reveal the correlation between intangible cultural heritage resources in the Yellow River Basin is of great significance to the protection and inheritance of intangible cultural heritage in the Yellow River Basin.[Method/process]Taking the intangible cultural heritage resources of the Yellow River Basin as the research object, the knowledge graph of the intangible cultural heritage resources in the Yellow River Basin was constructed in a top-down manner, and an intelligent question and answer system with user interaction as the core was constructed.[Result/conclusion]The intelligent question-and-answer system of intangible cultural heritage resources in the Yellow River Basin based on knowledge graph constructed in this study realizes the multi-dimensional knowledge discovery of intangible cultural heritage resources in the Yellow River Basin, and provides new ideas for the related research of intangible cultural heritage resources.

  • 科技期刊专题策划与传播的探索与实践——以《机械工程学报》为例

    Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》

    Abstract:【目的】探讨不同专题类型和传播方式对提升科技期刊学术质量和学术影响力的作用,为我国科技期刊学术引领和高质量发展提供借鉴。【方法】梳理《机械工程学报》近20年出版的专题,分类整理不同选题类型,并对融合出版趋势下专题的传播实践进行介绍。【结果】《机械工程学报》专题主要分为以纪念活动为主题、服务国家战略和重大项目、持续跟踪学科进展、及时报道新兴技术和学术思想的不同选题类型,并通过微信公众平台、专题线上分享会和线下主题论坛对专题进行二次传播,扩大专题影响。【结论】专题出版是科技期刊创新引领、汇聚优质稿源的重要方式。科技期刊应以专题为抓手,服务国家战略、服务行业、服务学者,并借助各种新技术手段,推进专题和期刊的高质量传播。

  • 媒介融合背景下的电视媒体与新媒体互联发展研究

    Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》

    Abstract:过去我国的媒体主要汇聚在电视媒体等群体之下,几乎所有传媒信息的发布都是以电视媒体为主,但是随着科技的发展和时代的进步,计算机互联网逐渐成为信息新闻等主要传播阵地,各种各样新式的传媒工具和传媒手段不断涌现,各类视频门户网站和平台等也对原有的传统传媒市场造成了冲击,在这样的环境下,为了实现我国文化传媒行业的蓬勃发展和多元化生长,将电视媒体与新媒体互联网逐渐结合起来就成为势在必行的一件事情。

  • 新时代广播电视媒体融合发展的思考和分析

    Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》

    Abstract:目前中国社会逐步迈入新时代,人们对生活的追求逐渐从单纯的物质层面上升到精神层面,更加注重生活的享受。其中广播电视媒体是人们较为热衷的享受方式,因而现代许多广播电视媒体都在寻求突破和发展。党的十九大提出要将媒体融合作为社会媒体发展的主旋律。许多媒体工作者也是立即展开了行动,努力跟上国家的号召。在这一过程中,许多广播电视媒体的融合发展战略逐渐被人们所知,使得更多的学者对这一内容进行专题的研究。本文在此背景下,从广播电视媒体的融合发展出发,着眼于目前的新时代,从生活中以及社会上寻找案例,先后阐述目前我国广播电视媒体中新旧媒体融合发展的意义、现存问题以及相关的解决策略,以期为现代广播电视等媒体的融合发展提供参考和借鉴,促进其更好地实施和演进,推动我国媒体事业的协调发展。

  • 基于区块链技术的新媒体内容信任机制建立

    Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》

    Abstract:新媒体时代,传媒行业已从传统纸媒发展转型到新媒体时期。与此同时,内容电商与大众日常生活的关联性也更大。电商行业在此背景下,为追求商业利益,通过创作新媒体内容来赚取流量费用,成为企业生存发展的重要一环。但因新媒体内容信任机制的严谨性不足,使得公众对新媒体的内容信任度不高,极大程度地损害了电商行业新媒体原创者的利益。由此,基于区块链技术的新媒体内容信任机制建立迫在眉睫。本文分析了基于区块链技术的新媒体内容信任机制概况,进一步分析了基于区块链技术的新媒体信任机制建立细节,以为新媒体内容信任机制建立提供可行性借鉴。

  • 新媒体环境下电视节目短视频化探究

    Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》

    Abstract:新媒体环境推动了电视节目的发展,本文在详细研究新媒体环境下电视节目发展现状的基础上,对目前电视节目短视频化的必然性进行分析;最后立足于新媒体环境,对电视节目短视频化的发展方向与方法作出研究。从本次研究结果可知,短视频化已经成为电视节目发展的必然趋势,因此,需要对电视节目的表现形式以及播放形式等进行创新,这样才能更好地适应新媒体环境的需求。

  • Comparative Study on ChatGPT Generation and Scholars Writing of Literature Abstracts: Taking the Field of Information Resource Management as an Example

    Subjects: Library Science,Information Science >> Information Retrieval submitted time 2023-08-28

    Abstract: Purpose/Significance Explore the similarities and differences between ChatGPT generation and Chinese paper abstracts written by scholars, and analyze the differences in content characteristics between the two, providing reference for AI generated academic paper detection and related research.  Method/Process Firstly, taking the field of information resource management as an example, we extracted 500 highly cited papers from library science, information science, and archival science in the past three years. Based on the obtained paper titles, we used the Prompt method to apply the ChatGPT tool to generate corresponding abstract texts and construct a dataset; Secondly, 9 machine learning and deep learning algorithms were used to classify and detect abstract texts generated by ChatGPT and written by scholars; Finally, analyze the similarities and differences between the two from multiple perspectives, including text features, topic models, and ROUGE evaluation, in order to reveal the similarities and differences between the two. Result/Conclusion Mainstream machine learning and deep learning algorithms trained on datasets can effectively distinguish whether abstracts are generated by AI or written by scholars, with BERT and ERNIE performing the best, while RF and Xgboost perform the best among machine learning algorithms. The number of abstract characters and sentences generated by ChatGPT is higher than that written by scholars, and the keywords are mostly template based transitional words; The themes of the two texts are mostly the same, but there are differences in themes such as "disciplinary system" and "digital humanities"; The quantitative analysis of ROUGE and cosine similarity indicates that the abstracts generated by ChatGPT have a significant "resemblance" rather than a "resemblance" to the abstract texts written by scholars.
     

  • 工作时间与工作绩效的非线性关系:一项元分析

    Subjects: Psychology >> Social Psychology submitted time 2023-03-28 Cooperative journals: 《心理科学进展》

    Abstract: Under the background of increasingly fierce market competition, employees are required to work longer hours under high-pressure management. However, there is little consistent evidence regarding the relations between work hours and job performance. To answer this question, based on the theory of limited self-control resources, we proposed a theoretical framework to explain the nonlinear relationship between work hours and job performance.In order to test theoretical hypotheses, we conducted a meta-analysis to examine the nonlinear relationship between work hours and job performance (task performance and contextual performance). A systematic review was conducted to synthesize empirical results about relationship between work hours and job performance. We collected articles relevant to work hours and job performance through CNKI, VIP, Web of Science, Wiley, ProQuest, EBSCO-Academic Search Premier and Science Direct (from inception to May 2022). Through literature retrieval and selection, in terms of the criteria for inclusion in the meta-analysis, 42 articles (64 effect sizes, 22843 subjects) were picked out as meta-analysis unit. After coding of data, meta-analysis was conducted with the metafor package in R (Version 4.2.0). The test for heterogeneity illustrated that there was significant heterogeneity, and also random effects model was an appropriate model for subsequent meta-analysis. Results of main effects showed that work hours had a significant inverted u-shaped correlation with task performance and contextual performance. Further, moderation analyses showed that the relationship between work hours and job performance were moderated by country context, industries types, age and gender. Specifically, compared with America and European countries, work hours had a stronger and significant inverted u-shaped relationship with task performance and contextual performance in China. Compared with labor-intensive and capital-intensive industries, in the knowledge-intensive industries, work hours had a stronger and significant inverted u-shaped relationship with task performance. Compared to older participants, work hours had a stronger and significant inverted u-shaped relationship with task performance in the younger participants. Compared to participants with a lower proportion of males, when the data came from participants with a higher proportion of males, work hours had a stronger and significant inverted u-shaped relationship with contextual performance.The research contributions are as follows. Firstly, existing studies have mainly conducted qualitative analysis to criticize the negative effect of long working hours system with regulative suggestions or used work hours as control variable to explore the linear correlation between work hours and job performance. Compared with existing studies, this study reveals and verifies the inverted U-shaped relationship between work hours and job performance (task performance and contextual performance) by meta-analysis. Secondly, previous studies have mainly explained the relationship and underlying mechanism from the perspective of physiological resources. Based on the theory of limited self-control resources, our study provides evidence that prolonged working hours, a specific aspect of work demands, which render individual psychological self-control resource loss and then make that work hours have a stronger and significant inverted u-shaped relationship with task performance and contextual performance. The findings broaden the theoretical mechanism underlie work hours and job performance. Thirdly, existing studies have rarely explored the moderating effect of the relationship between work hours and job performance, but this study explores the moderating effects of country context, industries types, age, gender and education level from macro, meso, and micro levels. Finally, this study finds that work hours have a significant inverted u-shaped correlation with task performance (the inverted U-shaped turning point was about 44.43 hours/week) and contextual performance (the inverted U-shaped turning point was about 47.88 hours), which can provid a scientific reference for standard hours stipulated by Labor Law in China (e.g. working hours should not exceed 44 hours/week) and warn that organizations should pay attention to the too-much-of-a-good-thing effect of work hours on job performance.

  • Species diversity of macrofungi in Gupo Mountain and adjacent areas of Guangxi, China

    Subjects: Biology >> Botany submitted time 2022-12-29 Cooperative journals: 《广西植物》

    Abstract:

    Gupo Mountain is adjacent to the Nanling Biodiversity Conservation Priority Area of China, which nurtures rich biological resources. To understand the diversity of macrofungal species in this area, a survey and collection of macrofungal resources in Guangxi Gupo Mountain Provincial Nature Reserve (abbreviate as Gupo Mountain) and adjacent areas was conducted by random tracking method, and combined morphological and molecular biological methods to identify. The results were as follows: (1) A total of 348 species macrofungal were found in this area, belonging to 175 genera in 69 families in 2 phyla, 6 orders and 17 classes, including 40 species of edible mushrooms, 42 species of medicinal mushrooms and 7 species of toxic mushrooms. (2) The analysis of the species composition of the region shows that the dominant families were nine families, including Agaricaceae, Boletaceae, Entolomataceae and Hymenochaetaceae, etc.; the dominant genera are eleven genera, including Amanita, Crepidotus, Crinipellis, Entoloma, etc. (3) There are two endemic genera in China, namely Fragiliporia and Zangia, and 11 endemic species, namely Coltricia crassa, Coltricia weii, Entoloma caespitosum, Entoloma praegracile, Entoloma subtenuicystidiatum, Fomitiporia tenuitubus, Fomitiporia torreyae, Fragiliporia fragilis, Perenniporia nanlingensis, Perenniporia subtephropora and Tremella cerebriformis. (4) Analysis of the funga composition of macrofungi in Gupo Mountain and adjacent areas revealed that the composition of families can be divided into cosmopolitan element (66.67%), pantropic element (17.39%) and north temperate element (15.94%); while the genera can be divided into seven elements, among which the cosmopolitan element is the most dominant (61.71%), and pantropic element (20.57%) and north temperate element (13.71%) rank second and thirdly. The rich species data provide basic information for the assessment of macrofungal species diversity and sustainable utilization of resources in Gupo Mountain and adjacent areas.

  • Nonlinear relationship between work hours and job performance: A meta-analysis

    Subjects: Psychology >> Management Psychology submitted time 2022-08-31

    Abstract:

    This research used meta-analysis to explore the nonlinear relationship between work hours and job performance based on 42 articles covering 64 effect sizes and 22843 subjects. Results showed that: (1) Work hours had a significant inverted u-shaped correlation with task performance and contextual performance. (2) Compared with America and European countries, work hours had a stronger and significant inverted u-shaped relationship with task performance and contextual performance in China. (3) Compared with labor-intensive and capital-intensive industries, in the knowledge-intensive industries, work hours had a stronger and significant inverted u-shaped relationship with task performance; (4) Compared to older participants, work hours had a stronger and significant inverted u-shaped relationship with task performance in the younger participants; (5) Compared to participants with a lower proportion of the males, when the data came from participants with a higher proportion of the males, work hours had a stronger and significant inverted u-shaped relationship with contextual performance. These results clarify the inverted u-shaped relationship between work hours and performance. In addition, these findings could provide a reference for standard hours stipulated by Labor Law in China (e.g. working hours should not exceed 44 hours/week) and warn that organizations should pay attention to the too-much-of-a-good-thing effect of work hours on job performance.

  • Optimization of chromosome preparation and chromosome numbers and ploidy in Hemiboea

    Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》

    Abstract: Chromosome numbers and genome ploidy are crucial basic information for evolutionary biology and genetics. Hemiboea, a genus of Gesneriaceae, with about 44 species, is mainly distributed in southern China, which have important medicinal and ornamental value. It is difficult to obtain suitable root tips for the chromosome preparation because of their small twining roots and small chromosomes. So far, only the chromosome numbers from three species of Hemiboea have been reported, while the chromosome numbers and ploidy of most species are unknown. The relationship between the pattern of chromosome number evolution and species evolution is also unclear. In this study, in order to explore the optimal conditions for chromosome preparation and the relationship between chromosome numbers and species evolution of Hemiboea, firstly, the root tips of six species (including H. subcapitata, H. longgangensis, H. longzhouensis, H. subacaulis var. jiangxiensis, H. follicularis, and H. yongfuensis), which were generated by the method of hydroponic rooting for cutting leaves based on the characteristics of leaf cuttage propagation of plants in the Hemboea, were used for the chromosome experiments. Then, we assessed the effects of different experimental conditions on chromosome preparation, and then, the conditions were optimized and the chromosome counting was performed. Finally, the evolutionary history of chromosome numbers in Hemiboea and the related genera including Anna and Loxostigma were traced based on the molecular phylogenetic relationships, and the evolutionary pattern of chromosome numbers and relationships with species evolution were studied. The results were as follows :(1) The optimal condition for karyotype preparation of the plants in Hemiboea are sampling during 9:30-10:00 am, dissociating for 10 min, and staining for 15 min. (2) All the six species of Hemiboea are diploid and with 32 chromosome numbers (2n=2x=32). (3) Except for a few species in the genus, the chromosome numbers of most species may be 2n=2x=32, and the variation of chromosome numbers may be caused by aneuploidy, which has no obvious relationship with the species evolution. This study shed light on chromosome preparation of Hemiboea and other groups with similar leaf regeneration characteristics, and provides implications for the classification and phylogeny of this group.

  • 泛中亚干旱区气候变化特征分析

    Subjects: Environmental Sciences, Resource Sciences >> Other Disciplines of Environmental Sciences, Resource Sciences submitted time 2021-03-02 Cooperative journals: 《干旱区研究》

    Abstract:利用Climatic Research Unit(CRU)1949—2018年最新0.5°× 0.5°网格点月均数据集,主要从EOF分解、小波分析的角度,讨论了泛中亚干旱区近70 a的气候变化特征。结果表明:(1)泛中亚干旱区近70 a降水以1.393mm·(10a)-1的速率呈增加趋势,夏季降水呈减少趋势,其余三季为增加趋势,其中冬季最明显[(0.834 mm·(10a)-1],同时冬季增温幅度也最大[0.360 ℃·(10a)-1];(2)降水距平场的EOF分析表明:泛中亚干旱区降水变化趋势呈整体一致性(降水第一模态),西南-东北反向变化特征(降水第二模态),由西向东“-+-”交替分布特征(降水第三模态),3个模态都存在显著的准3 a周期,第一模态还存在5~7 a和准12 a周期,第三模态存在准7 a变化周期;(3)温度距平场的EOF分析表明:温度距平变化表现出整体一致性(温度第一模态),东-西反向变化特征(温度第二模态),第一模态具有显著的准2 a、8~10 a周期,第二模态具有明显的2~4 a、准5 a周期。传统意义上的暖季并没有呈现出比较明显的增温多雨,冷季反而增温幅度更大,降水增加更明显。哈萨克斯坦中部、土库曼斯坦及蒙古国大部干旱化趋势明显,哈萨克斯坦东西两侧、我国新疆北部以及帕米尔高原的部分地方表现出显著的湿润化趋势,我国西北大部地区总体呈弱的湿润化趋势。随着亚洲夏季风的减弱,冷季降水增量高于暖季,这似乎说明亚洲冬季风对泛中亚干旱区降水的影响正在加大。本研究结果旨在加深对泛中亚干旱区气候变化特征的认识,为进一步防灾减灾、合理应对气候变化、坚持可持续发展战略提供科学依据。

  • 基于相对湿润度指数的西北地区春季第一场透雨研究

    Subjects: Geosciences >> Atmospheric Sciences submitted time 2021-02-28 Cooperative journals: 《干旱区地理》

    Abstract: 目前西北四省区春季第一场透雨标准的确定主要来自业务人员长期的工作经验积累,针 对此,利用陕西、甘肃、青海、宁夏 249 个气象站 1960—2016 年的日资料,通过分析西北地区相对湿 润度指数,结合潜在蒸散特征,确定了春季第一场透雨阈值。与已有研究相比,本方法充分考虑了 研究区各地相对湿润度指数的气候特征与各地的能量及水热平衡,理论依据明确,选用的指标能 充分反映各气象要素对气候干湿状况的综合影响,因此确定的透雨阈值稳定,月差异小。随后按 照这一指标分析西北地区春季第一场透雨特征,结果表明:该地区春季第一场透雨自东南向西北 逐步推进,陕南南部出现最早,主要在 3 月,关中、陕南北部出现在 3、4 月的概率之和>80%;甘肃河 东大部、宁夏大部、青海东部出现在 4、5 月的概率之和>70%;青海西部是出现最晚的区域,基本上 出现在 6 月。从出现日期的年代际变化看,甘肃河东出现时间最稳定,宁夏、陕西出现日期变化最 大,青海西部与甘肃河西较一致,介于二者之间;同时,甘肃河西、陕西关中、陕南春季第一场透雨 出现时间变晚趋势明显,其余地区变化趋势不明显。研究结果可供在透雨监测、预测中使用,也可 供在制定西北地区春季第一场透雨标准时参考,以便于区域气候评估使用。

  • 西北地区东部季风摆动区大气边界层高度对夏季风 活动和季风降水的响应特征

    Subjects: Geosciences >> Atmospheric Sciences submitted time 2021-01-06 Cooperative journals: 《干旱区地理》

    Abstract: 大气边界层高度影响着近地层能量、水分的发展变化,而季风摆动区边界层受夏季风活动 和季风降水影响很大,变化特殊,但其边界层高度的响应特征并不清楚。应用西北地区东部 5 站民 勤、榆中、平凉、银川和延安 2006—2016 年 5~9 月逐日 19 时每隔 10 m 高度高空加密观测资料,以 及民勤 2006—2016 年逐日 07 时探空规定层和特性层资料,结合地面逐日观测资料,对比计算多种 资料找到合适的边界层高度。进一步运用 NCEP、EC 再分析资料,分析夏季风对季风摆动区的影 响,得出边界层高度与夏季风、季风期降水影响的关系。结果表明:基于每隔 10 m 加密压温湿风探 空资料,确定了 9 点平均位温梯度法作为边界层高度的最佳计算方法,该区边界层高度 5~6 月较 高,7~9 月逐渐降低,5~9 月平均高度由非季风影响区的 2 600 m、季风摆动区的 1 800 m 逐渐降低 到季风影响区的 1 500 m 以下。边界层高度与地面相对湿度、地温和风场关系密切,湿度越大、风 速越大,边界层高度越低,相反,近地面地气温差越大,气温越高,吹西北风时,边界层高度越高。 在不受夏季风影响时,边界层高度较高,有夏季影响风时,边界层高度较低。夏季风持续时间越 长,边界层高度越低,当夏季风持续时间为 0 候、1~4 候和≥ 5 候时,边界层高度分别为 2 000 m 左 右、1 600~1 900 m 和 1 300~1 400 m。APO 季风强度指数与季风影响区边界层高度有显著的负相 关,APO 季风强度指数越大,季风影响区边界层高度越低。边界层高度与季风期降水性质、强度关 系较为密切,从大到小为无降水、对流性降水和稳定性降水;随着降水强度增强,边界层高度降低, 边界层高度中非季风影响区较高,季风摆动区次之,季风影响区最低。降水日数越多,边界层高度 越低。夏季风反过来对降低边界层高度,增多增强季风期降水起着积极作用。

  • 大豆类受体蛋白激酶基因 RLPK2 促进转基因拟南芥叶片的衰老

    Subjects: Biology >> Botany >> Applied botany submitted time 2020-11-27 Cooperative journals: 《广西植物》

    Abstract:大豆RLPK2 基因(GenBank 登录号: AY687391)是一个编码􀀁N-末端富含亮氨酸重复序列的类受体蛋白激酶基因。为分析大豆􀀁RLPK2 基因的功能,通过农杆菌介导法转化野生型拟南芥,构建了大豆􀀁RLPK2 基因过表达载体。该研究以野生型拟南芥和大豆􀀁RLPK2 基因过表达拟南芥植株为材料,分析了叶片衰老过程中叶绿素荧光参数、抗氧化酶活性及衰老相关基因表达量的变化。结果表明:(1)无论是野生型还是转基因拟南芥,随着叶片衰老进程的进行,光系统􀀁II(PSII)的最大光化学效率(Fv/Fm)、PSII 实际光化学效率(ΦPSII)、光化学淬灭系数(qP)和光合电子传递速率(ETR)均呈下降趋势,但后者下降趋势更明显;(2) 激发压(1-qP)在叶片衰老前期的变化较为平稳,后期则急剧增加,且转基因型比野生型拟南芥增加更明显;(3)在叶片衰老的各个时期,转基因拟南芥叶片丙二醛(MDA)含量均显著高于野生型,而超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均显著低于野生型;(4)实时荧光定量􀀁PCR 检测结果表明,RLPK2 转基因拟南芥中衰老标志基因􀀁ATSAG12,衰老关键转录因子􀀁ATNAP、ATWRKY6 和叶绿素降解关键酶编码基因ATACD1 等表达量显著上调。综上所述,大豆类受体蛋白激酶基因􀀁RLPK2 参与调控植物叶片衰老进程,其表达对叶片衰老起促进作用。

  • 半蒴苣苔复合群的多样性、地理分布和物种界限

    Subjects: Biology >> Botany >> Applied botany submitted time 2020-08-02 Cooperative journals: 《广西植物》

    Abstract:半蒴苣苔(Hemiboea subcapitata)为苦苣苔科半蒴苣苔属多年生草本植物。该种形态变异幅度大,曾包括多个变种;分布范围广,在多种类型的生境基质上皆有分布。本研究包括典型半蒴苣苔和与之近缘,且曾被归为其变种的翅茎半蒴苣苔(H. pterocaulis),以及我们新发现的与两者形态上分别最相近的另两种变异类型,对其关键形态、微形态的多样性进行了描述和比较;结合查阅标本、文献资料以及长期的野外追踪调查,统计分析了半蒴苣苔不同类型间的地理和生境分布;此外,通过DNA证据,初步重建各物种及变异类型间的系统发育和单倍型进化关系。研究结果表明:(1)通过茎、叶、花和开花物候等多性状组合能够区分半蒴苣苔复合群各类型。(2)半蒴苣苔复合群四类型间在叶表皮细胞形状和气孔分布等方面无甚区别;而在表皮毛的有无、复表皮层数、维管束数量和排布存上存在差别,或有助于分类。(3)典型半蒴苣苔广泛分布于我国中部至南部,向南延伸至越南北部,在石灰岩、丹霞等生境基质上皆有分布;翅茎半蒴苣苔仅分布于广西桂林市小部分区域的喀斯特山石灰岩基质上;而另两种变异类型则分布更为狭域,仅分布于桂林市永福县一个或相邻的数个喀斯特山石灰岩基质上。(4)DNA单倍型网络图分析表明,半蒴苣苔复合群四类型各自独享单倍型;但重建分子系统发育关系则表明,金钟山居群与翅茎半蒴苣苔聚为一支,两者亲缘关系最近但相互之间不成单系,金钟山居群是否为独立物种还需更多证据加以检验,而半蒴苣苔则与永福半蒴苣苔两者间互为单系,支持两者为独立物种。

  • 折力损伤自修复对干旱矿区小叶锦鸡儿 根系固土的影响

    Subjects: Environmental Sciences, Resource Sciences >> Basic Disciplines of Environmental Science and Technology submitted time 2018-11-08 Cooperative journals: 《干旱区研究》

    Abstract:为明确干旱矿区侵蚀发生后折力损伤对植物根系力学特性的影响及其受损后的自修复机制,利用HG100数显式推拉力计和自制便携式试验机台,对小叶锦鸡儿1~4 mm径级直根和侧根分支处未受损及受损自修复后的抗折力学特性进行研究。结果表明: 生长季初期,未受损小叶锦鸡儿根系极限抗折力与根径呈幂函数正相关,抗折强度与根径幂函数负相关,直根极限抗折力和抗折强度均大于侧根分支处; 折力损伤会明显抑制根系正常生长,小叶锦鸡儿根系受损自修复后,活性、生长量和保存率均低于平行对照,根径和根型均是影响这种抑制作用的重要因素,侧根分支处活性和保存率在受损后的减少程度显著大于直根; 小叶锦鸡儿根系受损自修复后,极限抗折力与抗折强度相比,生长季初期有所增加,但外力损伤会显著阻碍这种增长,导致其增长率显著低于平行对照,3个月后极限抗折力修复率为48.91%,抗折强度修复率为57.59%,说明根系受损后不会彻底丧失固土能力,通过自修复可以逐步恢复原有功能,但短期内自修复程度有限。直根极限抗折力修复率为60.55%,侧根分支处仅为36.34%,说明在同等外力荷载条件下,小叶锦鸡儿直根受损自修复能力显著大于侧根分支处,具备更强的再次抵御外力破坏的能力。