Subjects: Mathematics >> Algebra and Number Theory submitted time 2023-12-11
Abstract： Let be a k-algebra defined over a field k. This paper consider the two questions: If the tensor M⊗N on A is zero, under what situation is either M = 0 or N = 0? If the element, say m⊗n , in M⊗N is zero, under what situation is either m=0 or n = 0?
Subjects: Mathematics >> Algebra and Number Theory Subjects: Mathematics >> Discrete Mathematics and Combinatorics submitted time 2023-12-04
Abstract： Let $A_n$ be the Nakayama algebra of type $A$ with quadratic Jacobson radical to be zero and $X_n$ be the Nakayama algebra of type $A$ with quadratic Jacobson radical to be zero. In this paper, we consider the k-tensor $A_n otimes X_n$ and the classification of the indecomposable modules over $A_n otimes X_n$. Moreover, we provide a counting formula to compute the number of isoclasses of indecomposable $A_n otimes X_n$ -modules.
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Awaiting Review
Subjects: Library Science，Information Science >> Library Science submitted time 2023-08-27 Cooperative journals: 《图书情报工作》
Abstract： [Purpose/significance] Patent similarity detection assists the formulation of the national innovation strategy planning macroscopically, finds hotspots in China and all over the world, and deals with patent rogues in other countries. Microscopically, patent similarity detection provides support for patent inventors, patent examiners and patentees.[Method/process] A new method was proposed based on deep learning of Doc2Vec model, with patent corpus based on no data clearance of domain knowledge. Then typical patents were randomly selected to carry on similarity detection by this new method, and the results with traditional similarity detection models were compared.[Result/conclusion] According to experimental results, the new deep learning of Doc2Vec method and TFIDF model has similary results which both of the model's patent corpus all based on no data clearance of domain knowledge.The new method requires less professional skill in specific domain knowledge, and didn't require the process of data clearance. It can givesa new intelligent support tool for patent infringement and patent investigation, reduce the research threshold and workload, and improve service efficiency.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-28 Cooperative journals: 《中国科学院院刊》
Abstract： The development of data science is valuable to clarify the theoretical boundary of data science, and provides new possibilities and opportunities for the sustainable development of computing intelligence. Meanwhile, the development of computing intelligence and the emergence of new intelligence paradigms can offer new chance for applications of big data in various industries and fields. This paper discusses the connotation of data science, the development of computing intelligence, the new intelligence paradigm, and lists the key applications leading the development of data science and computing intelligence. Furthermore, based on the discussion during the 667th Xiangshan Science Conference, seven key problems of data science and computing technology are proposed, anticipating to attract attentions of both researchers and applications in related fields, grasping the opportunity of the era, and promoting sustainable development of data science and computing intelligence.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-03-01 Cooperative journals: 《中国全科医学》
Abstract： Background Klotho is closely related to the occurrence and development of kidney disease. Salt-sensitive hypertension（SSH）is often accompanied by kidney disease. At present，there are few reports on the role and molecules mechanism of klotho in renal injury in SSH. Objective To investigate the role and molecules mechanism of klotho in renal injury in SSH. Methods The rat glomerular mesangial cell line HBZY1 was selected as the experimental cells on June 15，2021， and the experimental cells were divided into the control group and the model group. The model of HBZY1 cell injury induced by NaCl 137 mmol/L and angiotensin Ⅱ（Ang Ⅱ）10-6 mmol/L was used to simulate the renal injury in SSH，and the cells were collected. The differences in the expression of klotho mRNA and protein were detected by real-time fluorescent quantitative PCR （qRT-PCR）and Western Blot. The interference vector and overexpression vector of klotho and the overexpression vector of angiotensin Ⅱ type 1 receptor（AT1R）were constructed. The klotho interference experiments were divided into five groups， including the control group，empty group，klotho-siRNA1 group，klotho-siRNA2 group and klotho-siRNA3 group； The klotho overexpression experiments were divided into three groups，including the control group，empty group and klotho overexpression group；The AT1R overexpression experiments were divided into three groups，including the control group，empty group and AT1R overexpression group. The constructed vectors were transfected into cells with verified transfection efficiency. After successful transfection，the experiment was divided into two parts. The first part of the experiment was to verify the renal protective effect of klotho，the experiment subjects were divided into four groups，including the control group，model group， klotho overexpression group and klotho interference group. The second part of the experiment was to explore whether the renal protective effect of klotho was related to AT1R，the experiment subjects were divided into three groups，including the model
group，klotho overexpression group and klotho+AT1R overexpression group. After successful transfection，the tests including cell viability detected by cell counting kit-8（CCK-8） method，reactive oxygen species（ROS）content detected by flow cytometry，malondialdehyde（MDA）and superoxide dismutase（SOD）content in cell supernatant detected by enzyme-linked immunosorbent assay（ELISA），interaction effect between kltho and AT1R detected by co-immunoprecipitation（Co-IP）. Results Compared with the control group，mRNA level and protein expression of klotho in the model group decreased in model group（ t=7.102，7.506； P=0.002，0.002），klotho-siRNA2 interference effect was more significant（ P<0.001）， the expression of klotho protein in the klotho overexpression group increased significantly（ P<0.001），the expression of AT1R protein in the overexpression group increased significantly（ P<0.001）. Effects of klotho on cell viability and oxidative stress injury：Compared with the control group，cell viability in the model group decreased（ P<0.001），intracellular ROS and MDA content increased（ P<0.001， P=0.004），and SOD content decreased（ P=0.041）；Compared with the model group，cell viability in the klotho overexpression group increased（ P<0.001），intracellular ROS and MDA content decreased and SOD content increased（ P<0.001， P=0.003， P=0.018）；Compared with the model group，cell viability in the klotho interference group decreased（ P<0.001），while intracellular ROS and MDA content increased and SOD content decreased（ P<0.001， P=0.002， P=0.001）. Effects of klotho on cell viability and oxidative stress injury through AT1R：Compared with the model group，cell viability increased（ P<0.001），intracellular ROS and MDA content decreased and SOD content increased（ P<0.001， P=0.024， P=0.007）in the klotho overexpression group；Compared with the klotho overexpression group，cell viability decreased（ P<0.001），ROS and MDA content increased and SOD content decreased（ P<0.001， P=0.001， P=0.002）in the klotho+AT1R overexpression group. Co-IP determined that there was an interaction between klotho and AT1R. Conclusion Klotho plays a protective role in renal injury in SSH by inhibiting oxidative stress injury through interaction with AT1R.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-01-28 Cooperative journals: 《中国全科医学》
Abstract：
Objective To investigate the role of Klotho in salt-sensitive hypertensive renal injury and its molecular mechanism. Methods HBZY1 cell injury model induced by NaCl 137 mmol/L and AngII 10-6 mmol/L was used to simulate salt-sensitive hypertensive kidney injury, and cells were collected. Real-time quantitative PCR (qRT-PCR) and Western Blot were used to detect the expression of Klotho mRNA and protein. The Klotho interference vector and overexpression vector were constructed, and the Angiotensinogen II receptor-1(AT1R) overexpression vector was transfected into cells. The transfection efficiency was verified by qRT-PCR and Western Blot. The following assays were performed after transfection. Cell proliferation activity was measured by CCK-8 assay, and ROS content was measured by Flow cytometry assay, the contents of malondialdehyde (MDA) and Superoxide dismutase superoxide dismutase (SOD) in the supernatant were detected by enzyme-linked immunosorbent assay (ELISA) , and the interaction between Klotho and AT1R was detected by co-immunoprecipitation (Co-IP) . Results Compared with the control group, the expression of Klotho mRNA and protein decreased in the model group (P<0.05). The KLOTHO-SIRNA2 interference effect was the best, and the expression of Klotho in the Klotho overexpression group and AT1R in the AT1R overexpression group increased, indicating the overexpression effect. Compared with the control group, the cell viability of the model group was decreased, the contents of ROS and MDA were increased, and the contents of SOD were decreased (P<0.05), the content of SOD decreased (P<0.05) , but the activity of klotho-treated cells decreased further, the content of ROS and MDA increased further, and the content of SOD decreased further (P<0.05). Compared with the model group, the Klotho overexpression group had higher cell viability, lower ROS and MDA contents and lower SOD contents (P<0.05) , while the Klotho + AT1R overexpression group had lower cell viability, higher ROS and MDA contents, the content of SOD decreased (P<0.05). CO-IP determines the interaction between Klotho and AT1R. Conclusion Klotho plays a protective role in salt-sensitive hypertensive renal injury by inhibiting oxidative stress injury through interaction with AT1R.
Subjects: Biology >> Bioengineering submitted time 2018-06-25 Cooperative journals: 《中国生物工程杂志》
Abstract：连续技术；工业生产；蛋白纯化；层析
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-21 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the role of receptor-interacting protein 140 (RIP140) in tumor-associated macrophages (TAMs) in the invasion and proliferation of hepatoma cells in vitro. Methods Western blotting, qRT-PCR and flow cytometry were performed to examine the effects of lentivirus-mediated RIP140 over-expression in mouse peritoneal macrophages (PMs). Western blotting, qRT-PCR and immunofluorescence staining were used to detect the expression of RIP140 in TAMs following stimulation of the PMs with hepatocellular carcinoma conditioned medium (HCM) for 24 h. The polarization index and the expression of NF-κB and IL-6 were detected using qRT-PCR in TAMs in HCM-stimulated PMs with or without RIP140 over-expression. Transwell assay and flow cytometry were used to estimate the cell invasion and apoptosis. HE staining and immunohistochemical staining were used to analyze the effects of RIP140-over-expressing macrophages on the growth and tumor formation of H22 cells in BALB/c nude mice. Results The lentivirus vector efficiently mediated RIP140 over-expression in mouse PMs. HCM stimulation significantly inhibited RIP140 expression in the TAMs and promoted their M2-like polarization. Over-expression of RIP140 in PMs suppressed the invasion and induced apoptosis of HCC cells. RIP140 over-expression inhibited HCM-induced M2 polarization and the activation of NF-κB/IL-6 axis in the TAMs, and RIP140-overexpressing TAMs obviously suppressed the growth of H22 cell xenograft in nude mice. Conclusion Over-expression of RIP140 in TAMs suppresses the growth and proliferation of hepatoma cells possibly by inhibiting M2 polarization of the TAMs.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effects of β-elemene in suppressing the proliferation and apoptosis of SGC7901 gastric cancer cells in vitro and explore the underlying mechanisms. Methods Using MTT assay, flow cytometry, and clonogenic survival assay, we assessed the effects of β-elemene on the viability, apoptosis, cell cycle distribution, and clonogenic survival of gastric cancer SGC7901 cells and gastric mucosal epithelial GES-1 cells. Western blotting was employed to determine the changes in the protein expression profiles in SGC7901 cells in response to β-elemene treatment. Results β-elemene significantly suppressed the cell viability and increased the apoptosis of SGC7901 cells, and these effects were less obvious in GES-1 cells. β-elemene decreased clonogenic survival of SGC7901 cells, increased the proportion of G2/M phase cells, decreased the expression of Bcl-2, and increased the expression of Bax and cleaved caspase-3. β-elemene did not obviously affect the expression of total p21-activated protein kinase 1 (Pak1) but decreased the level of phospho-Pak1 (Thr423) and phospho-ERK1/ 2 (Thr202/Tyr204) in SGC7901 cells. Conclusion β-elemene inhibits the proliferation and induces apoptosis of gastric cancer cells possibly by inhibiting Pak1/ERK signaling and regulating apoptosis-associated proteins such as Bcl-2 and Bax.
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