Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2023-11-16
Abstract： Objective: To compare urine proteome changes in the first two days after mating behavior in male rats.
Methods: The urine samples of Sprague-Dawley rats on the day of mating and the day after mating were collected and analyzed by non-label quantitative proteomics by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the differential proteins (FC > 1.5 or < 0.67) in the urine proteome were screened. P<0.050) protein function and biological pathways were analyzed.
Results: 43 different proteins were identified in the urine proteome between the day after mating and the day after mating. By searching the Uniprot database and Pubmed database and related literature reports, nearly two-thirds of the differential proteins were associated with spermatogenesis.
Conclusions: The urine proteome changed the day after mating compared to the day after mating, and some of the known functions of the changed proteins were associated with spermatogenesis.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2023-01-13
Abstract： In this study, we constructed a rat e-cigarette model and collected urine samples before, during, and after e-cigarette smoking in rats, that is, on days 0, 3, 12, 15, and 17, to explore e-cigarettes from the perspective of urine proteomics. In order to exclude the influence of individual differences, the experiment used a single rat before and after control for analysis, while the control group was set up to rule out differences caused by rat growth and development. The results showed that after smoking e-cigarettes under the same conditions, the differential proteins produced by rats had strong individual differences. We found that six experimental rats combined before and after controls identified fetuin-B, a biomarker of COPD, and annexin A2, which is recognized as a multiple tumor marker, among the differential proteins produced by rats on day 3 of e-cigarette smoking. Odorant binding proteins expressed in the olfactory epithelium were also found to be present in the urine proteome and were significantly upregulated in this study. We also found evidence that smoking e-cigarettes affects the immune system, cardiovascular system, respiratory system, etc. in both the resulting differential proteins and enriched signaling pathways, providing clues for further exploration of the mechanism of e-cigarettes on the human body.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-09-20
Abstract： The fear of startle is generated in the brain, and urine proteomics was used to explore whether this fear can be detected in the urine. The combination of natural enemy odor and sound stimulation were used to establish a rat model of startle. Urine samples were collected before and after startle and proteomic analysis was performed. The results showed that 22 differential proteins were identified after startle, and the biological pathways enriched in these differential proteins were related to neurotransmitter transport and glucose transmembrane transport, which may be the manifestations of nervous tension caused by startle. Before-after study in single rat was performed and found that there was one differential protein identified samely in five rats, in addition, 19 differential proteins were identified samely in four of five rats, and these proteins were associated with the change of nerve, motion, metabolism and blood pressure, which include catalytic subunits and regulatory subunits of glutamate-cysteine ligase, which realted to the function of startle. These results laid the foundation for the research of startle mechanism, and to find medicine for the treatment of psychological terror target provides a new method. At the same time, it fully illustrates the sensitivity of urine and opens up a new field for the exploration of urine.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry submitted time 2022-03-14
Abstract：
Objective This study focuses on the most important concern of surgeons - whether they resected all the tumors. Urine may reflect early changes associated with physiological or pathophysiological processes. Based on the above ideas, we have conducted some experiments to explore changes in the urine proteome urine proteomes between tumor-bearing mice to tumor-resected mice. Method The tumor-bearing mouse model was established with MC38 mouse colon cancer cell, and the mice were divided into the healthy control group, tumor complete resection group, and the tumor non-resection group. Urine was collected on the 7 days and 30 days after resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome and then analyze differential proteins and biological pathways. Results (1) 7 days after the tumor removal surgery between the complete resection group and the non-resection group, there are 20 differential proteins that can distinguish the two groups. The biological process includes circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, heterophilic cell#2;cell adhesion via plasma membrane cell adhesion molecules. (2) 30 days after the tumor removal surgery between the complete resection group and the non-resection group, there are 33 differential proteins that can distinguish the two groups. The biological process includes cell adhesion, complement activation, the alternative pathway, immune system process, angiogenesis. (3) There was no significant difference between the two groups at 30 days after the tumor removal surgery between the complete resection group and the healthy control group. Conclusion The changes of urine proteome can reflect tumor with surgical removal or not.
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Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Pharmacology submitted time 2022-03-09
Abstract：
[Objective] The drug consistency evaluation of atorvastatin by urine proteomic analysis.
[Methods] Intragastric administration rat models were established by atorvastatin from two companies. Urine samples were collected from rats before and after intragastric administration. Urine proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and the biological pathway of the differential proteins was analyzed by DAVID database.
[Results] The differential proteins were selected by comparing the after intragastric administration to before intragastric administration. A total of 116 differential proteins were identified in group A and 66 differential proteins in group B, and 24 proteins were identified in common between two groups. These differential proteins tend to be involved in biological pathways such as cell adhesion, negative regulation of endopeptidase activity and proteolysis.
[Conclusions] For two kind of atorvastatin, we can detect small differences in urinary protein between them, which confirms the sensitivity of urinary protein and suggests that urine proteomics has great potential for the Drug consistency evaluation.
Peer Review Status:Awaiting Review
Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2021-06-25
Abstract： "
Peer Review Status:Awaiting Review
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