Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： In this study, the ecological adaptation mechanisms of Anemone shikokiana in two distinct habitats, namely full-light hilltop scrub and shady mixed broadleaved-coniferous forest, were researched by Illumina high-throughput sequencing technology for leaves collected during the flowering stage. Moreover, EST-SSR molecular markers were developed based on SSR locus distribution characteristics. The results were as follows: (1) A total of 53 536 Unigenes sequences were obtained, of which 27 448 were successfully annotated. (2) 5 635 DEGs were obtained after filtering the low abundance genes, 1 600 up-regulated and 4 035 down-regulated genes comparing A. shikokiana in full-light hilltop scrub and in shady mixed coniferous forest. GO classification results showed that 2 460 DEGs were annotated to 2 533 tertiary entries. In addition, 1 051 DEGs were involved in 113 KEGG pathways. (3) The comprehensive analysis of the photosynthesis-antennal proteins pathway related genes revealed that the expression of lhca5 was significantly higher, while the expression of lhca1, lhca2 and lhca3 was significantly lower. Meanwhile, the comprehensive analysis of the flavonoid biosynthesis pathway related genes revealed that the expression of chs, c4h, f3’h, f3h, fls, ans, chi, ccoaomt and hct was significantly higher. (4) A total of 6 006 unigenes containing 7 146 SSRs were identified among 53 536 unigenes by using MISA software from the transcriptome data of A.shikokiana. In the identified SSRs, the dominant repeat motifs were single nucleotide repeats in 106 repetitive motif types. Among the 100 pairs of EST-SSR primers, a total of 68 pairs were effective and 11 pairs with polymorphism, and 24 polymorphic fragments were amplified. Overall, in this paper, the adaptation mechanisms of A.shikokiana in heterogeneous habitats were analyzed at the molecular level, and for the first time EST-SSR molecular markers were developed to fill the gap in this area, which would provide important molecular marker resource for the conservation and utilization of this species.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-26 Cooperative journals: 《广西植物》
Abstract： In this study, the ecological adaptation mechanisms of Anemone shikokiana in two distinct habitats, namely full-light hilltop scrub and shady mixed broadleaved-coniferous forest, were researched by Illumina high-throughput sequencing technology for leaves collected during the flowering stage. Moreover, EST-SSR molecular markers were developed based on SSR locus distribution characteristics. The results were as follows: (1) A total of 53 536 Unigenes sequences were obtained, of which 27 448 were successfully annotated. (2) 5 635 DEGs were obtained after filtering the low abundance genes, 1 600 up-regulated and 4 035 down-regulated genes comparing A. shikokiana in full-light hilltop scrub and in shady mixed coniferous forest. GO classification results showed that 2 460 DEGs were annotated to 2 533 tertiary entries. In addition, 1 051 DEGs were involved in 113 KEGG pathways. (3) The comprehensive analysis of the photosynthesis-antennal proteins pathway related genes revealed that the expression of lhca5 was significantly higher, while the expression of lhca1, lhca2 and lhca3 was significantly lower. Meanwhile, the comprehensive analysis of the flavonoid biosynthesis pathway related genes revealed that the expression of chs, c4h, f3’h, f3h, fls, ans, chi, ccoaomt and hct was significantly higher. (4) A total of 6 006 unigenes containing 7 146 SSRs were identified among 53 536 unigenes by using MISA software from the transcriptome data of A.shikokiana. In the identified SSRs, the dominant repeat motifs were single nucleotide repeats in 106 repetitive motif types. Among the 100 pairs of EST-SSR primers, a total of 68 pairs were effective and 11 pairs with polymorphism, and 24 polymorphic fragments were amplified. Overall, in this paper, the adaptation mechanisms of A.shikokiana in heterogeneous habitats were analyzed at the molecular level, and for the first time EST-SSR molecular markers were developed to fill the gap in this area, which would provide important molecular marker resource for the conservation and utilization of this species.
Subjects: Physics >> General Physics: Statistical and Quantum Mechanics, Quantum Information, etc. submitted time 2023-08-30 Cooperative journals: 《中国全科医学》
Abstract： Background There is growing evidence that pre-hospital use of metformin reduces mortality in patients with diabetes and sepsis；however，the effect of metformin on clinical outcomes in patients with diabetes and sepsis remains controversial. Therefore，there is a need for a systematic review and Meta-analysis of existing studies to further evaluate whether metformin can improve mortality and other clinical outcomes in patients with diabetes and sepsis. Objective To systematicallyevaluate the effects of pre-hospital metformin use on mortality，initial plasma lactate level and organ dysfunction in patients with diabetes and sepsis. Methods PubMed，Embase，Cochrane Library，Web of Science，Scopus，China Biomedical Literature Service System，Wanfang，CNKI and other databases were searched by computer for Chinese and English literature on the effects of pre-hospital metformin use on clinical outcomes in patients with sepsis and diabetes from inception to March 15，2023. The test group（MET group）involved adult patients with diabetes and sepsis received pre-hospital metformin use， and the control group（NM group）involved adult patients with diabetes and sepsis who did not receive pre-hospital metformin use. After screening，data extraction and literature quality evaluation were conducted by two researchers，Meta-analysis was performed using Revman5.3 software. Results A total of 12 papers were included in this study，involving 12 320 patients with diabetes and sepsis，with Newcastle-Ottawa Scale（NOS）scores of 7-8. The results of Meta-analysis showed that the mortality rate（OR=0.61， 95%CI=0.46-0.80，P<0.001）and the use of vasopressors（MD=0.83，95%CI=0.69-0.98，P=0.03）in MET group were lower than those in NM group；there was no significant difference in initial plasma lactate level（MD=0.31，95%CI=-0.12-0.75，P=0.16），serum creatinine level（MD=-0.81，95%CI=-0.48-0.13，P=0.25），initial blood glucose level（MD=32.94，95%CI=-10.12-76.01，P=0.13）and mechanical ventilation（MD=0.90，95%CI=0.77-1.06，P=0.23）between the MET group and NM group. Conclusion The pre-hospital use of metformin can reduce the mortality of patients with diabetes and sepsis，and reduce the use of vasoppressors in corresponding patients，providing updated evidence that metformin can reduce the mortality of patients with diabetes and sepsis. However，whether it can reduce the sepsis severity score and reduce the maximum dose of norepinephrine in patients with diabetes and sepsis remains to be further studied.
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