Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2024-04-29 Cooperative journals: 《中国全科医学》
Abstract： Background At present，there are few studies on the stability of warfarin anticoagulation therapy. The main evaluation method is SAMe-TT2R2 score，but it has obvious limitations and is not applicable to non-white population. Previous studies have shown that platelet distribution width and left atrial diameter are closely related to TTR. Whether they can be incorporated into the existing scoring system and improved is worthy of clinical reflection. Objective To explore the predictive value of modified SAMe-T-PDW2-LAD2 score for the percentage of time within therapeutic range（TTR）of common international normalized ratio（INR）for the stability of warfarin anticoagulation therapy. Methods A total of 164 patients with persistent nonvalvular atrial fibrillation who were treated with warfarin anticoagulation in Jiujiang First People's Hospital from January 2021 to June 2023 were selected as the research objects. TTR ≥ 65% was defined as high quality anticoagulation. TTR<65% was defined as substandard，that is，the stability of warfarin anticoagulation therapy was not high-quality. According to the above criteria，the patients were divided into two groups：TTR ≥ 65% group（TTR ≥ 65%，46 cases）and TTR<65% group（TTR<65%，118 cases）. The modified SAMe-T-PDW2-LAD2 score retained the four parameters of gender，age， comorbidities and combined medication in the previous SAMe-TT2R2 score，and the two parameters of race and smoking were deleted. ＂ Increased platelet distribution width（PDW） ＂ and ＂ increased left atrial diameter（LAD） ＂ were replaced by 2 points respectively. The above parameters were collected and compared between the two groups. Binary Logistic regression analysis was used to explore the influencing factors of stable TTR target in warfarin anticoagulation therapy. Receiver operating characteristic（ROC）curves of SAMe-TT2R2 score and modified SAMe-T-PDW2-LAD2 score were plotted to evaluate the TTR target stability of warfarin anticoagulation therapy. Results Single factor analysis showed that there were significant differences in age，combined with heart failure，combined with hyperlipidemia，smoking，increased PDW and increased LAD between the two groups（P<0.05）. Binary Logistic regression analysis showed that age（OR=0.882，95%CI=0.812-0.958，P=0.003）， increased PDW（OR=0.443，95%CI=0.282-0.697，P<0.001），and increased LAD （OR=0.031，95%CI=0.001-0.853， P=0.040）were the influencing factors of achieving stable TTR target on warfarin anticoagulation therapy. There were significant differences in TTR and TTR reaching the target rate between groups with different scores of SAMe-TT2R2 score（P<0.05）. The TTR of scores 6 and 7 groups was higher than that of scores 2，3，4 and 5 groups（P<0.05）. The TTR target rate in the score 7 group was higher than that in the score 2，3，4，5，and 6 groups（P<0.05）. There were significant differences in TTR and TTR reaching the target rate among different score groups of modified SAMe-T-PDW2-LAD2 score（P<0.05）. The TTR of scores 3，4，5，6，and 7 groups was lower than that of scores 0，1，and 2 groups（P<0.05）. The TTR target rate in the integral 2 group was higher than that in the integral 0，1，3，4，5，6，and 7 groups（P<0.05）. ROC curve results showed that the area under the ROC curve（AUC）of SAMe-TT2R2 score for evaluating the stability of warfarin anticoagulation therapy TTR target was 0.803（95%CI=0.737-0.868），the best cut-off value was 5.5，the sensitivity was 1， and the specificity was 0.508. The AUC of modified SAMe-T-PDW2-LAD2 score was 0.814（95%CI=0.751-0.877），the best cut-off value was 2.5， the sensitivity was 1，and the specificity was 0.576. Conclusion Age，increased PDW and increased LAD are the influencing factors of achieving stable TTR target in warfarin anticoagulation therapy. There are many limitations of SAMe-TT2R2 score in evaluating the stability of warfarin anticoagulation therapy. The modified SAMe-T-PDW2-LAD2 score has better clinical application value in evaluating the stability of warfarin anticoagulation therapy.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-12-11 Cooperative journals: 《中国全科医学》
Abstract： Background Atherosclerosis is the primary cause of cardiovascular and cerebrovascular diseases worldwide，and inflammation is a current research focus，with NOD-like receptor 3（NLRP3）being the most intensively studied inflammasome. GLP-1 receptor agonists have shown anti-atherosclerotic effects，but the underlying mechanisms remain unclear. Objective To investigates the mechanism of liraglutide in antagonizing oxidized low-density lipoprotein（ox-LDL）induced endothelial cell injury. Methods Human umbilical vein endothelial cells（HUVECs）were cultured，and HUVEC with blank serum was served as the control group，100 μg/mL ox-LDL treated HUVECs for 48 hours was served as the model group. Liraglutide was added in concentrations of 100 nmol/L，200 nmol/L，and 400 nmol/L to the HUVECs treated with ox-LDL for 24 hours，forming low，medium，and high concentration liraglutide groups，respectively. Cell proliferation rates were calculated using the CCK-8 method. Pyroptotic cell morphology was observed by using scanning electron microscopy. Lactate dehydrogenase（LDH）activity was measured. The expression levels of interleukin （IL） 1β and IL-18 were detected using enzyme-linked immunosorbent assay（ELISA）. Western blotting was used to assess the expression levels of NLRP3，apoptosis-associated speck-like protein containing a CARD（ASC），caspase-1，gasdermin D（GSDMD），and N-terminal GSDMD（N-GSDMD）. Results Cell proliferation rate in the model group and both low and medium concentration liraglutide groups were lower than the control group，while the rate in all liraglutide-treated groups were higher than the model group（P<0.05）. Scanning electron microscopy showed obvious pyroptosis in the model group cells，which was significantly reduced in all liraglutide-treated groups.LDH activity in the model group and the low concentration liraglutide group was higher than the control group，while it was lower in all liraglutide-treated groups compared to the model group（P<0.05）. IL-1β level in the model group and the low concentration liraglutide group was higher than the control group，whereas IL-1β levels in the medium and high concentration liraglutide groups was lower than the model group（P<0.05）. IL-18 level in the model group was higher than the model group，while level in all liraglutide-treated groups was lower than the model group（P<0.05）. The expression levels of NLRP3，ASC，caspase-1，GSDMD，and N-GSDMD in the model group were higher than the control group. In the low concentration liraglutide group，ASC and caspase-1 levels were higher than the control group，whereas in the medium concentration group，NLRP3 and ASC levels were lower than the model group. In the high concentration group，NLRP3，ASC，and caspase-1 levels were lower than the model group（P<0.05）. Conclusion Liraglutide significantly inhibits NLRP3 inflammasome activation in endothelial cells induced by ox-LDL，and can inhibit endothelial cell pyroptosis，with anti-atherosclerotic effects.
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-12-11 Cooperative journals: 《中国全科医学》
Abstract： Background At present，the main methods for diagnosing coronary heart disease are coronary contrast#2;enhanced computed tomography and angiography，of which coronary contrast-enhanced computed tomography is limited in clinical application due to its poor accuracy and some patients' inability to perform because of rapid heart rate. While coronary angiography，which is the "gold standard"，is also hampered in its promotion due to its invasive nature and the difficulty in popularizing in primary care institutions. It is particularly important to use some simple and easily accessible indicators to participate in the diagnosis of coronary heart disease. This study explores the diagnostic value of circadian variability of systolic blood pressure combined with SDNN in coronary heart disease，with the aim of proposing a simple and practical new predictive method. Objective To explore the diagnostic and predictive value of circadian variability of systolic blood pressure combined with SDNN for coronary heart disease，in order to provide non-invasive methodological reference for evaluating and predicting the diagnosis of coronary heart disease. Methods From January 2018 to December 2022，246 patients who were definitely diagnosed as coronary heart disease by coronary angiography and had Holter monitor examination results and blood pressure monitoring records before coronary angiography were selected as the research objects，and the general baseline data of coronary heart disease and non coronary heart disease were compared. Using the diagnosis of coronary heart disease as the observation endpoint，the correlation between various parameters included in the observation and endpoint events was analyzed，as well as the possible influencing factors related to endpoint events. The predictive value of circadian variability of systolic blood pressure combined with SDNN for the diagnosis of disease was further analyzed. Results The baseline data comparison between the coronary heart disease group and non coronary heart disease group showed statistically significant differences in age，SDNN，and circadian variability of systolic blood pressure（P<0.05）. Correlation analysis showed that age and circadian variability of systolic blood pressure were positively correlated with the diagnosis of coronary heart disease，LVD and SDNN were negatively correlated with the diagnosis of coronary heart disease. Binary Logistic regression analysis showed that age，LVD，EDV，CO，SDNN，and circadian variability of systolic blood pressure were influential factors in the diagnosis of coronary heart disease. The AUC of SDNN for diagnosing coronary heart disease was 0.690（95%CI：0.620，0.760），with sensitivity and specificity of 0.753 and 0.615，the corresponding optimal cutoff value was 128.5. The AUC of circadian variability of systolic blood pressure for diagnosing coronary heart disease was 0.908（95%CI：0.869，0.948），with sensitivity and specificity of 0.847 and 0.896，and the corresponding optimal cutoff value was 14.61%. The AUC of circadian variability of systolic blood pressure combined with SDNN for diagnosing coronary heart disease was 0.914（95%CI：0.875，0.953），with sensitivity and specificity of 0.827 and 0.917，and the corresponding optimal cutoff value was 0.721. Conclusion Circadian variability of systolic blood pressure combined with SDNN has good clinical application value in predicting and diagnosing coronary heart disease.
Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》
Abstract：中国自古以来就是一个农业大国，农业在我国的发展中起着举足轻重的作用，随着改革开放的不断推进，三农问题一直伴随着中国的发展，三农问题尚未完全解决，这是转型期发展中需要解决的根本问题。研究发现，随着科学技术的飞速发展，多媒体技术已经成为人们获取信息的一种方式，农业电视节目在促进三农问题的解决方面发挥着重要的作用，这在提高信息传播效率的同时，也导致了农业电视节目分类的下降。在这种背景下，农业电视节目必须顺应时代发展的潮流，根据公众的真实需求，进行传播模式的创新，为进一步提高节目的影响力和受众关注度，为实现我国农业发展和全体人民共同富裕的目标做出更大贡献。本文简要介绍了新媒体背景下农业电视节目面临的困境，分析了整合新的传播技术创新农业电视节目传播模式的优势，最后提出了农业电视节目传播模式创新的建议，希望能够最大程度上吸引农业电视节目的受众，促进农业电视节目的进一步发展。
Subjects: Library Science，Information Science >> Library Science submitted time 2023-10-08 Cooperative journals: 《知识管理论坛》
Abstract： [Purpose/significance] From the perspective of industry chain, this paper takes virtual reality technology as an example, constructs VR patent industry chain corpus, and explores the technical theme, research and development hotspot and future development trend of China VR patent. [Method/process] First of all, this paper used Python to crawl the patent text in VR field and got effective corpus through data cleaning. Secondly, combining IPC classification number and K-means clustering algorithm, this paper constructed and validates VR patent industry chain. In addition, based on TF-IDF algorithm and LDA theme model, we identified the core technology themes and their comprehensive strength, technology research and development hotspots and future trends of China VR patents from the perspective of production chain. [Result/ conclusion] At present, the proportion of patents in each link of China VR industry chain is unbalanced. The upstream link is the most popular, followed by the downstream link, and the weakest link is the midstream link. In terms of theme mining, the upstream hot spot is software development, the midstream hot spot is film and television production, and the downstream hot spot is medical, educational and entertainment applications. In terms of future trends, the upstream of the industrial chain will be dominated by technologies such as electronic digital data processing, optical components, image communication, etc., the midstream will be dominated by technologies such as vehicle components, power devices, damping devices, etc., and the downstream will be dominated by technologies such as indoor games, medical diagnosis, identification, etc.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： Collisionless magnetic reconnection, which converts the magnetic energy into the kinetic energy of plasma particles via the heating or acceleration, has been believed widely to be able to explain various eruptive phenomena such as solar flares and geomagnetic storms. However, the microphysical mechanism of anomalous resistivity in the collisionless magnetic reconnection is still an unsolved fundamental problem. Among the many physical mechanisms of anomalous resistivity formation, chaos-induced resistivity based on the chaos of the charged particle orbits near the magnetic neutral point is not the most popular formation mechanism, but its microscopic physical picture is the clearest. This paper first briefly reviews the early research and physical model of the chaos-induced resistivity in collisionless magnetic reconnection region, introduces the recent research progress of the chaos-induced resistivity, and expounds the future research direction of the chaos-induced resistivity.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： Compared to solar radio bursts, interplanetary (IP) radio bursts with lower radiation frequencies are generally believed to originate in the interplanetary space far from the low corona. The cutoff by Earth's ionosphere makes the ground-based observations for it impossible. Parker Solar Probe (PSP), launched by the National Aeronautics and Space Administration (NASA) to explore the Sun with the closer heliocentric distance than any previous spacecraft, provides an opportunity to study the low-frequency radio burst. The radio spectrometer it carries can observe radio radiation in the frequency range of 10 kHz--19.17 MHz. PSP can approach the radiation source region of the IP type III radio burst. Therefore, it has an unprecedented advantage to observe the interplanetary radio bursts. This paper reviews the studies about type III radio bursts observed by PSP so far. These studies include the occurrence rate, polarization, scattering, cutoff frequency, possible radiation mechanisms, and related radiation source regions of radio bursts and so on. Finally, future research prospects are discussed.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： The radio emission mechanisms, especially the coherent radio emission mechanisms of radio bursts, are one of the most complicated and controversial topics in astrophysics. Due to the complex influence of multiple physical factors, theoretical studies of coherent radio emission mechanisms are very difficult. There has been a constant controversy between the two types of coherent emission mechanisms, i.e., the plasma emission and the electron cyclotron maser emission, for a long time. In recent years, people have been trying to apply the fully-kinetic Particle-in-Cell (PIC) numerical simulations to the study of coherent radio emissions, and have made some positive progress. In addition to reviewing some existing problems and difficulties in this topic, this paper will focus on the research of PIC simulations for the coherent radio emissions from space plasmas and the main progress in recent years.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： The heliospheric radio emissions are the strongest radio emissions phenomenon in the solar system, with a radiation power of at least 10$^{13}$\;W, which can provide important physical information of high energy electron beam and magnetic plasma structure near the heliospheric boundary. Since the first detection by the Voyager spacecraft in 1983, those radio emissions have widely and continuously attracted much attention from researchers. There are generally two types of the heliospheric radio emissions: instantaneous or drifting emission with relatively high frequency, and continuous emission or non-drifting emission with relatively low frequency. Usually, both types of emissions start from about 2\;kHz. For the drifting emission, it has the characteristic of drifting to high frequency, the drifting rate is about 1--3\;kHz/yr, the frequency range is 1.8--3.6\;kHz, and the duration is about 100--300\;days. For the non-drifting emission, it has no obvious frequency drift, the frequency range is 1.8--2.6\;kHz, and the duration is about 3\;yr. It is generally believed that the heliospheric radio emissions are related to shock. In this paper, the possible source region of the radio emissions, the emission mechanisms, and the source of shock related to the emissions are introduced. Furthermore, the existing scientific problems and the future perspectives on the research of heliospheric radio emissions are discussed.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： Radio bursts are ubiquitous in the cosmic plasma. Solar radio emission mainly comes from the outer atmosphere of the sun. It is an induced radiation phenomenon generated by the interaction between energetic electrons and solar atmospheric plasma. Different dynamic spectra of solar radio bursts (SRBs) contain physical information of the plasma structure and state in the radiation source region. Therefore, the radiative mechanism of radio bursts has always been the object of research. There are two kinds of coherent radiation mechanisms related to solar radio bursts: one is the plasma radiation mechanism based on electron Langmuir frequency; the other is the electron cyclotron maser (ECM) radiation mechanism based on the electron cyclotron frequency. Although these two radiation mechanisms were proposed almost at the same time, based on the understanding of the coronal environment and the ECM mechanism at that time, the ECM radiation mechanism did encounter some difficulties in explaining SRBs. Until 1979, Wu $\&$ Lee introduced the relativistic effect and used the ECM radiation to explain the earth's Auroral Kilometric Radiation (AKR). Since then, the ECM emission has attracted wide attention. Considering some difficulties in applying the ECM emission mechanism to SRBs, we proposed a series of modified models in recent years. Firstly, the cutoff in the energy spectrum of the power-law electrons can effectively drive the ECM instability without relying on the anisotropic distribution of electron velocity. Secondly, considering the influence of Alfv\'{e}n wave perturbations which are prevalent in space and celestial plasmas, a self-consistent ECM emission mechanism excited by energetic electron beams is developed. On this basis, this paper summarizes the application of the ECM emission mechanism in traditional SRB phenomena from type I to V and microwave SRBs in recent years.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： Kinetic Alfv\'en waves (KAWs) are dispersive Alfv\'en waves with a perpendicular wavelength comparable to the ion gyroradius or the electron inertial length. Due to the scale of waves matching to the kinetic scale of particles, KAWs can play an important role in the heating and acceleration of particles in solar and space plasmas. Therefore, KAWs are often regarded as a candidate for the coronal heating. In this paper, the excitation and dissipation mechanisms of KAWs in the solar atmosphere are investigated systematically. Based on the coronal plasma environment, this paper introduces several excitation mechanisms of KAWs: anisotropic temperature instability, field-aligned current instability, electron beam instability, density inhomogeneity instability and resonant mode conversion. The dissipation mechanisms of KAWs in the solar atmosphere are introduced, and their effects on the plasma heating in the sunspot, coronal loop and coronal plume are also discussed. This review not only provides a reasonable theoretical basis for understanding the driving mechanism, dynamic evolution characteristics and wave-particle interaction of KAWs in the solar atmosphere, but also helps to reveal the microscopic physical mechanism of energy storage and release, as well as particle heating in the corona.
Subjects: Astronomy submitted time 2023-07-14 Cooperative journals: 《天文学报》
Abstract： As well known, more than 99\% of the observable matter in the universe is plasma and the plasma astrophysics studies various physical processes and phenomena occurring in cosmic plasmas, from small-scale collective interaction processes and energy transforming mechanisms in particle kinetics to the state of large-scale structure of cosmic objects and their eruptive phenomena. The present paper reviews the important role of the plasma astrophysics in the development of the modern astronomy as well as the formation of the modern plasma cosmology based on the history of the cosmic evolution, the formation of large-scale structures, and eruptive phenomena of cosmic objects. In addition, the unique function of satellite in situ exploring researches in space plasmas to act as the natural laboratory for the plasma astrophysics is further elaborated.
Subjects: Library Science，Information Science >> Information Science submitted time 2023-04-01 Cooperative journals: 《图书情报工作》
Abstract： [Purpose/significance] Technological innovation is an important driving factor for economic development and transformation. We attempt to empirically analyze the technological innovation effect of China government big data policy, which will help to promote the national innovation driven development strategy and the deep integration of data elements and technical elements,realizing linkage innovation and open innovation.[Method/process] Based on the action plan for big data development and the establishment of the national comprehensive test area for big data, this paper took the panel data of 31 provinces in China from 2000 to 2019 as the research sample, and used Propensity Score Matching method to analyze the tendency score matching between the experimental group and the control group. In addition, this paper utilized the Difference in Differences method to double difference the matched samples, and used the method of variable replacement to test the robustness, so as to explore the relationship between the government big data policy and technological innovation.[Result/conclusion] By solving the endogenous problem of public policy and the non observability of virtual facts, we have found that government big data policy can promote technological innovation.
Subjects: Library Science，Information Science >> Information Science submitted time 2023-04-01 Cooperative journals: 《图书情报工作》
Abstract： [Purpose/significance] In the context of data-driven paradigm, this paper reveals the internal relationship between the open data state of government portal website and the behavior state of its main body, and promotes the effect and process of government data opening.[Method/process] This paper used the crawler method to grab the open data sets in the Shanghai government data portal, and then did correlation analysis and Stepwise regression analysis on the index variables of each data set in turn, and screened out the variables with high correlation degree. At the same time, we further carried out PLS regression test on the variables with significant relationships, and ultimately drew the internal relationship between the state of government open data and the state of its main body's behavior.[Result/conclusion] In the process of government data opening, the subject behavior of government departments has a greater impact on public subject behavior than the object characteristics of data itself. Among the factors affecting the public rating, the sequence from high to low is:government openness and secrecy, machine readability of Data Format, the first opening time of government, the timeliness of government openness. The level of government openness and secrecy has a significant negative impact on the score of government open data; the first opening time of government, the timeliness of government openness, and the machine readability of data format have a significant positive impact on the public score.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-28 Cooperative journals: 《中国科学院院刊》
Abstract： With the call of scientists getting engaged in science popularization, science popularization in the context of combination of scientific research and popularization becomes a necessary requirement of evaluating science popularization of scientists. Meanwhile, many relevant policies, regulations, and guidelines have been issued in recent years. This study analyzes the history of the combination of scientific research and popularization and corresponding requirements in those documents that being issued by governments, while discusses the necessity and problems of combination of scientific research and popularization. Some suggestions on how to improve scientists’ science popularization capacity in the context of combination of scientific research and popularization are proposed.
Subjects: Library Science，Information Science >> Information Science submitted time 2022-06-14
Abstract：
Abstract：[Purpose/Significance] As an important part of digital China, digital village and digital government complement each other. Digging out the policy correlation between the two and exploring the similarities and differences in policy content will help promote China's digital transformation and realize efficient government service, scientific decision-making model and refined urban and rural governance. [Method/Process] Based on policy informatics theory, the text contents of digital village policy and digital government policy are compared and studied, and the coding results of the two policies are calculated by using grey relational degree models. [Result/Conclusion] Digital village policy and digital government policy have the highest correlation in the dimension of digital technology construction, indicating that they have the most similar policy needs for digital technology construction. In the dimension of economic construction, the correlation degree is the lowest, and the demand of digital village policy in economic construction is much higher than that of digital government policy, indicating that the development of digital rural needs the support of economic foundation."
Peer Review Status:
Awaiting Review
Subjects: Astronomy >> Astrophysical processes submitted time 2021-05-27 Cooperative journals: 《天文研究与技术》
Abstract：GECAM是专门针对引力波伽马暴的研究机遇而提出的中国科学院空间科学（二期）先导专项卫星任务。GECAM卫星通过数传、遥测以及北斗短报文三个通道下行触发、事例、并道、工程以及短报文等多种类型的数据，数据预处理过程需要对这些数据进行快速正确处理，以满足科学家对天文事件数据正确性和时效性的要求。本文针对GECAM卫星数据处理时效性要求高、数据连续完整、多信道数据融合处理以及触发事件数据切分等特点，对科学数据处理流程和天文警报信息处理流程进行了设计，概述了关键核心算法的思路与实现方法。卫星发射入轨后，数据预处理软件运行良好，正确性和及时性指标满足要求，为后续爱因斯坦探针（EP）卫星、中法天文卫星（SVOM）等空间天文卫星开展数据预处理工作提供了参考。
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To establish a method for amplifying specific 16S rDNA fragment of algae related with drowning and test its value in drowning diagnosis. Methods Thirty-five rabbits were randomly divided into 3 the drowning group (n=15), postmortem water immersion group (n=15, subjected to air embolism before seawater immersion), and control group(n=5, with air embolism only). Twenty samples of the liver tissues from human corpses found in water were also used, including 14 diatom-positive and 6 diatom-negative samples identified by microwave digestion-vacuum filtration-automated scanning electron microscopy (MD-VF-Auto SEM). Seven known species of algae served as the control algae (Melosira sp, Nitzschia sp, Synedra sp, Navicula sp, Microcystis sp, Cyclotella meneghiniana, and Chlorella sp). The total DNA was extracted from the tissues and algae to amplify the specific fragment of algae followed by 8% polyacrylamide gelelectrophoresis and sliver-staining. Results In the drowning group, algae was detected in the lungs (100%), liver (86%), and kidney (86%); algae was detected in the lungs in 2 rabbits in the postmortem group (13% ) and none in the control group. The positivity rates of algae were significantly higher in the drowning group than in the postmortem group (P<0.05). Of the 20 tissue samples from human corps found in water, 15 were found positive for algae, including sample that had been identified as diatom-negative by MD-VF-Auto SEM. All the 7 control algae samples yielded positive results in PCR. Conclusion The PCR-based method has a high sensitivity in algae detection for drowning diagnosis and allows simultaneous detection of multiple algae species related with drowning.
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究仔猪出生后10~20 d，早期断奶仔猪小肠谷氨酸转运载体基因表达情况与哺乳仔猪的差异。试验分别从40头不同母猪的仔猪中各选出体重相近，10日龄的“杜×长×大”三元杂交仔猪1头，共40头仔猪，随机不配对分为2组，每组20头仔猪，对照组（哺乳组）为哺乳仔猪，随母猪喂养；试验组（断奶组）为断奶仔猪，隔离断奶饲养；试验期10 d。饲养结束，每组随机取12只仔猪，宰杀取空肠和回肠，测定谷氨酸转运载体兴奋性氨基酸转运载体1（EAAC1）蛋白质表达情况和游离氨基酸含量。结果显示，断奶显著降低了仔猪空肠和回肠EAAC1（57和73 ku）及其相关蛋白谷氨酸转运联合蛋白（GTRAP3-18）（50 ku）的蛋白质和基因表达量（P<0.05）。断奶提高了仔猪空肠游离谷氨酸和总氨基酸含量，却降低了仔猪回肠游离谷氨酸和总氨基酸含量，差异显著（P<0.05）。结果提示，早期断奶降低EAAC1和GTRAP3-18的蛋白质含量，这可能与早期断奶仔猪遭受营养谷氨酸缺乏导致的肠道氨基酸吸收转运障碍有关。
Hits
Hits
Downloads
Comment
YES