Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2024-02-19 Cooperative journals: 《中国全科医学》
Abstract： Background Ischemic heart disease is recognized as the most common cardiovascular disease with the highest mortality rate worldwide，resulting in an increasing burden of disease on our population. Conducting trend and predictive analysis of the changing burden of disease is an important topic of concern for health care policy. Objective To compare and analyze the burden of disease and risk factors of ischemic heart disease in China and globally from 1990 to 2019，to predict the trend of change，and to provide a basis for carrying out the prevention and treatment of ischemic heart disease. Methods Based on the Global Burden of Disease Study 2019 (GBD2019)，the log-linear model in Joinpoint was used to analyze the changing trends of the burden of disease of ischemic heart disease in China and globally from 1990 to 2019，and trend tests were performed to predict the burden of disease of ischemic heart disease in China from 2020 to 2035 with the help of R 4.3.0. Results From 1990 to 2019，the standardized incidence rate，standardized mortality rate，and standardized DALY rate of ischemic heart disease in China were lower than the global level，but all of them showed an overall increasing trend，while all of them showed a decreasing trend globally. The high incidence，mortality，and DALY rates of ischemic heart disease in China and globally are mostly in the elderly population，and the overall burden of ischemic heart disease in China is expected to decrease from 2020 to2035 (AAPC for morbidity，mortality，and DALY rates are -0.400，-1.167，and -1.318，respectively; P<0.001)，with greater decreases in all indicators in men than in women in both China and globally. Conclusion The situation of ischemic heart disease prevention and treatment in China is severe，and the disease burden is particularly heavy in the male and elderly populations; the disease burden caused by disability is high; high systolic blood pressure and high-density lipoprotein are important risk factors. In the future，we should pay attention to the increasing trend of disease burden of ischemic heart disease in China，and focus on strengthening preventive interventions in men and the elderly population.
Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》
Abstract：计算机网络技术的飞速发展、互联网技术的快速革新,为新媒体技术的普及奠定了坚实、稳定的基础。如今,新媒体技术已经成为出版领域的中坚力量,传统出版业不能再固守原有的发展思维,而是应当主动与新媒体融合,赢得良好的发展机遇,探索与时俱进的革新路径。本文主要围绕着如何推进传统出版与新媒体技术融合发展这一问题开展研究,从与新媒体相关的各项内容入手,探索融媒体思维引导下的出版领域的发展路径。
Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》
Abstract：5G时代技术改革让融媒体新闻的传播获得了新的转机,在满足社会发展逐渐变革的环境下,围绕受众的市场需求,以常态化直播与新型手段当作融媒体的发展与生存趋势。本文主要分析5G时代融媒体的背景与特点,5G时代融媒体发展机遇和挑战,以及基于5G时代融媒体的直播报道趋势,从而为国家的稳定发展提供保障。
Subjects: Medicine, Pharmacy >> Clinical Medicine submitted time 2023-07-26 Cooperative journals: 《中国全科医学》
CHEN, Yanyan
SHI, Min
WANG, Yi
FU, Jianfang
ZHANG, Ying
LIU, Xiangyang
ZHANG, Weiqing
TA ,Shengjun
LIU, Liwen
LI, Zeping
ZHOU, Jie
LI, Xiaomiao
Abstract： Background In recent years，studies have demonstrated the use of ultrasound speckle tracking techniques for the early evaluation of subclinical left ventricular（LV） systolic function in patients with type 2 diabetes mellitus（T2DM）. In addition，long-term exposure to hyperglycemia severely damages the microvascular system. However，the interaction between diabetic microvascular complications and subclinical LV systolic function has not been fully understood. Objective To investigate the relationship between subclinical LV systolic function and diabetic microvascular complications in patients with T2DM. Methods A total of 150 patients with T2DM who were admitted to the Department of Endocrinology，the First Affiliated Hospital of Air Force Medical University from June to December 2021 were selected in the cross-sectional study and all underwent conventional echocardiography，pulsed tissue Doppler echocardiography and two-dimensional speckle tracking echocardiography（2D STE）. The 2D global longitudinal strain（GLS） of LV was obtained to assess the subclinical LV systolic function. Diabetic microvascular complications were defined as combined diabetic retinopathy，diabetic kidney disease or peripheral neuropathy. The subjects were divided into the T2DM alone group（T2DM alone group，n=76），the T2DM with 1 complication group〔T2DM+C（1） group，n=37〕 and the T2DM with 2 to 3 complications group〔T2DM+C（2 to 3）group，n=37〕 according to the cumulative number of microvascular complications. The general demographic characteristics and biochemical indices of patients in the three groups were compared. The relationship between microvascular complications and subclinical LV systolic function was assessed by using Spearman correlation analysis and Logistic regression analysis. Results GLS was lower in the T2DM+C（1） and T2DM+C（2 to 3） groups than in the T2DM alone group （P<0.05）. Spearman rank correlation analysis showed that the number of diabetic microvascular complications involved was negatively correlated with GLS（rs=-0.349，P<0.001）. Logistic regression analysis showed that after adjustment for multiple factors of gender，age，duration of diabetes，hypertension，glycosylated hemoglobin，fasting glucose，blood creatinine，heart rate，urinary microalbumin，and LV fraction shortening，GLS was decreased in the T2DM+C（1） group〔OR=0.744，95%CI（0.601，0.920）〕 and the T2DM+C（2 to 3） group〔OR=0.707，95%CI（0.525，0.952）〕 compared with the T2DM alone group （P<0.05）. Multivariable Logistic regression analysis based on hypertension stratification showed that after adjustment for the same confounding factors described above，diabetic microvascular complications remained independently associated with reduced GLS〔with hypertension：OR=0.556，95%CI（0.321，0.963）；without hypertension：OR=0.739，95%CI（0.558，0.979），P<0.05〕 in T2DM patients with or without hypertension. Conclusion The LV systolic function may already be impaired in hospitalized T2DM patients with diabetic microangiopathy. The increased number of microvascular complications involved was closely related to subclinical LV systolic dysfunction，independently of hypertension.
Subjects: Library Science，Information Science >> Information Science submitted time 2023-04-01 Cooperative journals: 《图书情报工作》
Abstract： [Purpose/significance] The evaluation index system of smart library development is constructed by combining quantitative and qualitative methods,which provides scientific standard and quantitative tool for the evaluation practice of smart library. [Method/process] Based on literature research and object-oriented analysis of smart library, the index system was preliminarily formulated. Then, aiming at the preliminary indicators,Delphi method was used tocarry out expert consultation, andthe final evaluation index systemwas determined. Meanwhile, Analytic Hierarchy Process (AHP) and expert survey were used to calculate and determine the weight of evaluation indexes.Finally,based on the evaluation index system,the development strategy and operation of smart library were analyzed. [Result/conclusion] The evaluation index system of smart library development was constructed, which consisted of 4 dimensions, 12 first-level indexes and 50 second-level indexes. Under the perspective of evaluation, the smart representation and developmentstrategy of smart library were provided.
Subjects: Other Disciplines >> Synthetic discipline submitted time 2023-03-28 Cooperative journals: 《中国科学院院刊》
Abstract： The European Union (EU) is one of the origins of the global semiconductor industry, but due to its structural defects in the semiconductor value chain, it has encountered a crisis of chip shortages and supply cuts since 2021. The European Chips Act drafted by EU proposes a path for semiconductor ecological construction, crisis intervention regulatory measures, and outlines a blueprint for a “national system for the chip industry with EU characteristics”. These factors are of speculative significance and reference value for China to balance the relationship between the government and the market, subsidies and competition, key construction and inclusive support in the process of high#2;intensity investment and supply chain construction in the field of semiconductor chips.
Subjects: Psychology >> Developmental Psychology submitted time 2023-03-28 Cooperative journals: 《心理科学进展》
Abstract： Feedback-seeking behavior refers to individuals proactively seek feedbacks about their performance, role position or job targets from other individuals or their leaders. This study systematically summarizes the existing literatures in aspects of the concept, measurement, influencing factors and consequences of feedback-seeking behavior, at the same time highlights the effects of Chinese indigenous culture. We identify that future study should try to improve the concept of feedback-seeking behavior, develop new measurement which fits into new cultural and economic background, expand research level to organization and team level, and take Chinese special organizational situation into consideration to make further discussion toward feedback-seeking behavior.
Subjects: Psychology >> Social Psychology submitted time 2023-03-27 Cooperative journals: 《心理学报》
Abstract： In the current age, employees are expected to work proactively in workplaces where the organizational structure is flatter and knowledge updating becomes faster than ever. There is a growing consensus on the importance of proactive behaviors from both scholars and practitioners. Proactive behavior, defined as “an anticipatory action that employees take to impact themselves and/or their environments” is thought to be self-initiated, future-oriented and change-oriented. Evidence showed that employees’ proactive behavior is beneficial for both individual performance and organizational competitiveness. Existing research regarding the impacts of proactive behavior mainly focuses on effectiveness of employees’ own proactive behaviors, such as promoting their job satisfaction or organizational commitment. However, no one is an isolated island. In a team or an organizational environment, employees’ attitudes and behaviors will inevitably be affected by their coworkers. Accordingly, this study focused on coworker proactive behavior and discussed its effectiveness in stimulating employees’ job performance. Drawing upon social learning theory, we hypothesized employee autonomous motivation will mediate the relationship between coworker proactive behavior and employee job performance. We further assumed that whether an employee possesses highly proactive personality determines the odds that employees might be motivated by their proactive coworkers. These hypotheses were tested with a field sample of supervisor-employee dyads and an experimental study. For the field study, we conducted a three-wave research design and achieved 209 valid samples from employees and their supervisors in two high-tech enterprises based in Beijing and Hebei provinces. At Time 1, employees assessed their proactive personality and their coworkers’ proactive behavior, and provided their demographic information. At Time 2 (one month after Time 1), employees were asked to report their autonomous motivation. At Time 3 (two months after Time 1), supervisors provided performance evaluation of employees who engaged in the survey. For the experimental study, we recruited 86 full-time workers from a high-tech company located in Hebei province via its internal communication channel. These respondents were separated into two groups randomly, namely coworkers with high proactive behaviors (N = 74) and coworkers with low proactive behaviors (N = 76). First, respondents were asked to finish a measure of their proactive personality and report their demographics. Then, they were given a scenario, one of which depicted a situation where coworkers were proactive (or not proactive). Finally, after reading the scenario, respondents finished a manipulation check of coworker proactive behavior and reported the degree of their autonomous motivation. We applied confirmatory factor analysis, regression analysis and mixed model via SPSS 23 and Mplus 7.4 to analyze the data. Empirical results supported our hypotheses and indicated the following: (1) Coworker proactive behavior had a significant positive effect on employee autonomous motivation; (2) Employee autonomous motivation played a mediating role in the relationship between coworker proactive behavior and employee job performance; (3) Employee proactive personality played a moderating role in the relationship between coworker proactive behavior and employee autonomous motivation, such that coworker proactive behavior was positively related employee autonomous motivation when employees’ proactive personality was high, while such relationship became negative when employees’ proactive personality was low. This study makes several theoretical and managerial implications. First, by investigating the effectiveness of coworker proactive behavior, this study offers a new insight in proactive behavior research by incorporating the influence of coworker into consideration. Second, by examining the mediating role of employee autonomous motivation, this study enhances our understanding of how coworker proactive behavior translates into employee job performance. Third, by exploring the contingent role of employee proactive personality, this study shows the boundary condition under which employees are more likely to be motivated by their proactive coworkers.
Subjects: Nursing >> Nursing submitted time 2023-03-06
Abstract： Objective To improve the cost accounting of hospital Central Sterile Supply Department (CSSD), explore the operation decision of CSSD based on cost management perspective, and study the cost accounting method of disinfection supply service in line with the actual situation of the hospital. Methods A study on cost accounting methods for operation cost of CSSD was conducted based on activity-based costing theory. Unit costs of services from recycling of disinfection sterilization package, cleaning and disinfection, high and low temperature sterilization were calculated precisely. Calculation of unit cost was standardized and refined. Results In the information tracing management system, the calculating formula was generated to perform the cost statistics monthly. Labor costs accounted for the highest proportion in each process of CSSD. Conclusion Scientific cost management can provide an important basis for managers to scientifically manage the cost of CSSD for internal transfer pricing and external service pricing strategies .
Peer Review Status:
Awaiting Review
Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Other Disciplines of Agriculture, Forestry,Livestock & Aquatic Products Science submitted time 2023-02-17 Cooperative journals: 《智慧农业（中英文）》
Abstract： Plant micro-phenotype mainly refers to the phenotypic information at the tissue, cell, and subcellular levels, which is an important part of plant phenomics research. In view of the problems of low efficiency, large error, and few traits of traditional methods for detecting kernel microscopic traits, Micro-CT scanning technology was used to carry out precise identification of micro-phenotype on 11 varieties of maize kernels. A total of 34 microscopic traits were obtained based on CT sequence images of 7 tissues, including seed, embryo, endosperm, cavity, subcutaneous cavity, endosperm cavity and embryo cavity. Among the 34 microscopic traits, 4 traits, including endosperm cavity surface area, kernel volume, endosperm volume ratio and endosperm cavity specific surface area, were significantly different among maize types (P-value<0.05). The surface area of endosperm cavity and kernel volume of common maize were significantly higher than those of other types of maize. The specific surface area of endosperm cavity of high oil maize was the largest. The endosperm cavity of sweet corn had the smallest specific surface area. The endosperm volume ration of popcorn was the largest. Furthermore, 34 traits were used for One-way ANOVA and cluster analysis, and 11 different maize varieties were divided into four categories, of which the first category was mainly common maize, the second category was mainly popcorn, the third category was sweet corn, and the fourth category was high oil maize. The results indicated that Micro-CT scanning technology could not only achieve precise identification of micro-phenotype of maize kernels, but also provide supports for kernel classification and variety detection, and so on.
Subjects: Library Science，Information Science >> Library Science submitted time 2022-04-02
Abstract：今年是武汉大学信息管理学院建院百年华诞（前身是创建于1920 年的武昌文华大学图书科），这标志着中国图书馆学教育已走过百年的发展历程。 回首历史，在充分肯定图书馆学教育对中国图书馆事业发展的贡献的同时，也应正视如今图书馆学教育的困顿，直面图书馆学教育的两难选择。 困顿的核心问题是图书馆学教育工作者及图书馆从业人员如何认识和处理职业、专业、事业的关系，承担起中国图书馆学教育应有的责任与使命。
Peer Review Status:Awaiting Review
Subjects: Management Science >> Development and Management of Human Resources submitted time 2021-12-06
Abstract： " In the current age, employees are expected to work proactively in workplaces where the organizational structure is flatter and knowledge updating becomes faster than ever. There is a growing consensus on the importance of proactive behaviors from both scholars and practitioners. Proactive behavior, defined as “an anticipatory action that employees take to impact themselves and/or their environments” is thought to be self-initiated, future-oriented and change-oriented. Evidence showed that employees’ proactive behavior is beneficial for both individual performance and organizational competitiveness. Existing research regarding the impacts of proactive behavior mainly focuses on effectiveness of employees’ own proactive behaviors, such as promoting their job satisfaction or organizational commitment. However, no one is an isolated island. In a team or an organizational environment, employees’ attitudes and behaviors will inevitably be affected by their coworkers. Accordingly, this study focused on coworker proactive behavior and discussed its effectiveness in stimulating employees’ job performance. Drawing upon social learning theory, we hypothesized employee autonomous motivation will mediate the relationship between coworker proactive behavior and employee job performance. We further assumed that whether an employee possesses highly proactive personality determines the odds that employees might be motivated by their proactive coworkers. These hypotheses were tested with a field sample of supervisor-employee dyads and an experimental study. For the field study, we conducted a three-wave research design and achieved 209 valid samples from employees and their supervisors in two high-tech enterprises based in Beijing and Hebei provinces. At Time 1, employees assessed their proactive personality and their coworkers’ proactive behavior, and provided their demographic information. At Time 2 (one month after Time 1), employees were asked to report their autonomous motivation. At Time 3 (two months after Time 1), supervisors provided performance evaluation of employees who engaged in the survey. For the experimental study, we recruited 86 full-time workers from a high-tech company located in Hebei province via its internal communication channel. These respondents were separated into two groups randomly, namely coworkers with high proactive behaviors (N = 74) and coworkers with low proactive behaviors (N = 76). First, respondents were asked to finish a measure of their proactive personality and report their demographics. Then, they were given a scenario, one of which depicted a situation where coworkers were proactive (or not proactive). Finally, after reading the scenario, respondents finished a manipulation check of coworker proactive behavior and reported the degree of their autonomous motivation. We applied confirmatory factor analysis, regression analysis and mixed model via SPSS 23 and Mplus 7.4 to analyze the data. Empirical results supported our hypotheses and indicated the following: (1) Coworker proactive behavior had a significant positive effect on employee autonomous motivation; (2) Employee autonomous motivation played a mediating role in the relationship between coworker proactive behavior and employee job performance; (3) Employee proactive personality played a moderating role in the relationship between coworker proactive behavior and employee autonomous motivation, such that coworker proactive behavior was positively related employee autonomous motivation when employees’ proactive personality was high, while such relationship became negative when employees’ proactive personality was low. This study makes several theoretical and managerial implications. First, by investigating the effectiveness of coworker proactive behavior, this study offers a new insight in proactive behavior research by incorporating the influence of coworker into consideration. Second, by examining the mediating role of employee autonomous motivation, this study enhances our understanding of how coworker proactive behavior translates into employee job performance. Third, by exploring the contingent role of employee proactive personality, this study shows the boundary condition under which employees are more likely to be motivated by their proactive coworkers.
Peer Review Status:Awaiting Review
Subjects: Geosciences >> Geography submitted time 2020-01-06 Cooperative journals: 《干旱区地理》
Abstract：在全球变化的背景下，植被生产力发生了一系列的变化，如何定量的评估中国西北干旱区气候变化和人类活动对植被生产力的影响，对于应对气候变化，促进“一带一路”生态建设以及美丽中国建设具有重要的意义。以新疆为研究区，以植被净第一性生产力（NPP）作为评价指标，分析了2001—2016年气候变化和人类活动对植被恢复和退化的影响。结果表明：（1） 从2001—2016年，植被NPP有明显变化趋势的面积占植被覆盖区总面积的34.02%，其中30.58%的面积呈现恢复趋势，3.44%的面积呈现退化趋势，NPP平均每年增加634 Gg C·a-1(Gg=109 g)。（2） 由人类活动和气候变化引起植被恢复的面积占植被NPP变化总面积的42.03%和30.58%；在上述两个区域，NPP平均每年增加量分别为319 Gg C·a-1和59 Gg C·a-1。由人类活动和气候变化引起植被退化的面积占NPP变化总面积的57.63%和19.45%；其中，在上述两个退化区域，NPP平均每年分别减少68 Gg C·a-1和7 Gg C·a-1。（3） 不同植被类型中，人类活动对农作物、荒漠、草地、高山植被的恢复作用大于退化作用，对森林、灌丛、沼泽的退化作用大于恢复作用；气候变化对沼泽的退化作用大于恢复作用，对其他6种植被类型的恢复作用大于退化作用。总体上，人类活动是影响新疆植被恢复和退化的主要原因。
Subjects: Geosciences >> Atmospheric Sciences submitted time 2019-11-14 Cooperative journals: 《干旱区研究》
Abstract： 潜在蒸散发在区域水量平衡、干旱程度评价、农作物需水量等方面的研究中具有重要的作用。然而，潜在蒸散发的空间化处理一直以来都是相关研究面临的一个挑战。基于新疆地区1960—2017年66个气象站的观测数据，通过一种简单的参数方程，实现了潜在蒸散发（ET0）的空间化处理。研究结果表明：① 简化参数方程中的2个重要参数a，c的空间分布呈现一定的规律，参数a在空间上呈现东南高，西北低的特点；参数c则随海拔的增加而增大。② 简化参数方程与Penman-Monteith方法相比，拟合结果在日、月和季节尺度上的R2值均大于0.90，且R2值随时间尺度的增加而增加。③ 将简化参数方程的拟合结果与CRU数据和MOD16A2数据进行对比发现：简化参数方程与Penman-Monteith方法拟合的R2值较高，拟合效果和偏差指标表现更佳，而CRU、MOD16A2数据拟合的R2值较低。简化参数方程所获得的潜在蒸散发精度高且空间分辨率更高（500 m×500 m），是一种适用于新疆地区潜在蒸散发估算的简便有效的方法。
Subjects: Psychology >> Applied Psychology Subjects: Computer Science >> Computer Application Technology submitted time 2018-05-09
Abstract： By using social media big data, this study explores the role of regional factors in the process of haze influence. Based on keyword frequency, we analyzed weibo original content of users from Beijing(Chaoyang district) and Chengdu, after delete hot events which greatly influence people’s emotion. We introduced of regulating variable (area) and found that there is interaction of areas and haze(PM2.5), haze positively related with negative emotion in Beijing and haze negatively related with negative emotion in Chengdu. There are regional differences in the influence of haze (PM2.5), which can be related to the lifestyle and historical culture of the two cities.
Peer Review Status:Awaiting Review
Subjects: Computer Science >> Integration Theory of Computer Science submitted time 2018-05-02 Cooperative journals: 《计算机应用研究》
Abstract： With respect to the problems of Multiple Attribute Decision-Making in which the attribute values are in the form of interval-valued Hesitant fuzzy numbers（IVHFN）and attributes are associated with each other. A novel decision-making method based on interval-valued Hesitant fuzzy weighted Heronian mean(IVHFWHM) operator is proposed. According to the IVHFN’s operational laws and Heronian Mean(HM) operator, interval-valued Hesitant fuzzy Heronian mean(IVHFHM) operator and IVHFWHM operator are proposed. Then, the IVHFHM’s properties of permutation invariance, idempotent, monotonicity, boundedness and parameter symmetry are studied. A multiple attribute decision-making model based on IVHFWHM operator is constructed. Finally, A numerical example of MADM problem is provided to illustrate the effectiveness and feasibility of the proposed operator.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of exenatide on chemotactic migration of adipose-derived stem cells (ADSCs) and confirm that Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 migration pathway. Methods ADSCs were isolated, cultured, identified by flow cytometry and induced to differentiate in vitro. RTCA xCELLigence system was used to analyze the effect of exenatide on ADSC proliferation. The effects of exenatide at different concentrations, AMD3100 (CXCR-4 antagonist), and CCG-1423 (Rho GTPase antagonist) on chemotactic migration of ADSCs were tested using Transwell assay. The expression of CXCR-4 in exenatide-treated ADSCs was measured by flow cytometry and Western blotting. Active Rhopull-down detection kit was used to detect the expression of Rho GTPase. Laser confocal microscopy was used to observe the formation of stress fibers in ADSCs with different treatments. Result Exenatide treatment for 24 h had no significant effect on ADSC proliferation. Exenatide obviously promoted chemotactic migration of ADSCs in a concentration-dependent manner, and this effect was blocked by either AMD3100 or CCG-1423. Both flow cytometry and Western blotting showed that exenatide dose-dependently up-regulated CXCR-4 expression in ADSCs. Western blotting showed that the expression of Rho GTPase was related to SDF-1/CXCR-4 pathway and laser confocal microscopy revealed that the formation of stress fibers in ADSCs was related to SDF-1/CXCR-4/ Rho GTPase pathway. Conclusion Exenatide promotes chemotactic migration of ADSCs, and Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 pathway.
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of liraglutide, an analogue of glucagon-like peptide-1, on the proliferation and migration of cardiac microvascular endothelial cells (CMECs) and explore the mechanism. Methods In vitro cultured CMECs of SD rats were purified by differential adhesion method and identified immunocytochemically using CD31 antibody and factor VIII. MTT assay was performed to assess the proliferation of the first-generation cells exposed to different concentrations (0-1000 nm/L) of liraglutide. Western blotting was used to detect the activation of PI3K/Akt and MAPK/ERK signaling pathways. BrdU fluorescent labeling and scratch assay were performed to observe the proliferation and migration of CMECs following liraglutide treatment, and PI3K/Akt and MAPK/ERK pathway inhibitors LY294002 and PD98059, respectively, were used to further confirm the role of these signaling pathways in regulating the proliferation and migration of CMECs. Results Immunocytochemical staining demonstrated a proportion of double positive cells exceeding 95%. The cells exhibited a logarithmic growth 48 h after plating. Liraglutide exposure concentration-dependently promoted the proliferation of CMECs with the optimal concentration of 100 nmol/L (P<0.05). Liraglutide exposure of the cells for 24 h significantly increased the levels of intracellular phosphorylated Akt and ERK (P<0.05), but pretreatment of the cells with Akt and ERK signaling pathway inhibitors 1 h before liraglutide obviously reversed such effect (P<0.05). BrdU and scratch assay showed that 100 nmol/L liraglutide significantly promoted the proliferation and migration of CMECs (P<0.05), but such effects were obviously suppressed by Akt and ERK inhibitors (P<0.05). Conclusion Liraglutide promotes the proliferation and migration of CMECs in vitro via PI3K/Akt and MAPK/ERK signaling pathways.
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract： Abstract: [Objective] To explore the function of the transcription factor CodY on flagellar motility, bacterial virulence in Listeria monocytogenes. [Methods] The codY gene on the L.monocytogenes chromosome was knocked out by homologous recombination and the strain of the deleted codY gene was successfully complemented by the codY gene construct; the results were evaluated by observation of the motility of flagellar movement, the transcriptional expression of flagellum-related genes with RT-qPCR. The effects of wild-type strain EGDe and CodY-deficient strain on bacterial hemolytic activity, semi-lethal dose of Helicoverpa armigera and the main virulence factor LLO(coding gene hly) and virulence regulatory protein PrfA(coding gene prfA) transcription expression profiles were compared. [Results] Compared with the wild-type strain, the CodY-deficient strain showed a significant reduction in the flagellar movement, the hemolytic activity, as well as the transcriptional expression of flagellum-related genes and the major virulence genes hly and prfA(P≤0.01); while the semi-lethal dose on Helicoverpa armigera (Hübner) was increased 5.8 fold. [Conclusion] The CodY plays an important role in flagellar motility and transcription regulation of bacterial virulence in Listeria monocytogenes.
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